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Искусственный интеллект в войне растений

Введение.

Культурные растения, которые мы выращиваем для получения урожая, являются одними из ключевых объектов сельского хозяйства. Однако на полях и огородах также произрастают сорные растения (сорняки), которые конкурируют с культурными за ресурсы. В природе тоже идут войны и это одна из них (рис. 1).

Рисунок 1. Война растений
Рисунок 1. Война растений

Химическое оружие растений.

Как известно, оружейный арсенал один из наиболее значимых факторов на войне. У растений таковым выступают токсичные вещества, которые они используют для подавления роста конкурирующих видов. Данное явление называется аллелопатия, а вещества, оказывающие соответствующее действие - аллелохимикаты. Термин аллелопатия был введен австрийским ботаником Гансом Молишем в 1937 году в книге “ The Influence of One Plant on Another: Allelopathy” [1]. Однако со временем концепция аллелопатии расширилась и стала включать в себя взаимодействие между любыми живыми организмами, осуществляемое за счет секретируемых ими химических веществ [2]. И давайте не забывать о том, что мы, люди, также принимаем участие в этой войне. В наших интересах получить урожай, обеспечивая культурным растениям благоприятные условия для их прорастания, угнетая рост сорняков. Для этого агрономы используют гербициды. Что такое гербициды? Гербициды - это синтетические аналоги аллелохимикатом. Таким образом, обе группы оказывают негативное влияние на растения. Такое свойство веществ называют фитотоксичностью. Механизм фитотоксического действия основан на нарушении различных жизненно важных процессов растений. К их числу относятся фотосинтез, синтез аминокислот, липидов и прочее (рис. 2).

Рисунок 2. Механизмы фитотоксичности
Рисунок 2. Механизмы фитотоксичности

Но как мы можем действовать на тот или иной физиологический процесс растения? На самом деле, каждому из них соответствует определенный биохимический путь, состоящий из ряда молекул-участников. Аллелохимикаты (как и гербициды) могут связываться с некоторыми из них так, что нарушается соответствующий физиологический процесс. Как правило, наиболее уязвимыми для воздействия в биохимических каскадах являются белки (ферменты, рецепторы и др.). Поэтому именно они и являются мишенью для большинства аллелохимиатов. Более того, существует список основных мишеней, ассоциированных с фитотоксичностью (табл. 1).

Таблица 1. Мишени фитотоксичности

Механизм фитотоксичности

Мишень

Ингибиторы синтеза аминокислот

EPSP (5-енолпирувилшикимат-3-фосфатсинтаза)

ALS (ацетолактатсинтаза)

Ингибиторы синтеза липидов

Ацетил-КоА-карбоксилаза

Ингибиторы фотосинтеза

Белки фотосистемы II

Ингибиторы азотистого обмена

Глютаминсинтетаза

Регуляторы роста

Ауксиновые рецепторы

Разрушители клеточной мембраны

Протопорфириногеноксидаза

Ингибиторы синтеза пигментов

DOXP (1-дезокси-2-ксилулоза-5-фосфат)

Рассмотрим пример аллелохимиката - феруловую кислоту. Ее фитотоксическое действие обусловлено ингибированием (замедлением) синтеза белка D1, необходимого для протекания фотосинтеза (рис. 3) [3]. Логично ожидать, что нарушение этого процесса приведет к снижению синтеза углеводов и, как следствие, к смерти растения “от голода”. Однако в действительности губительное действие ингибиторов фотосинтеза связано с тем, что их действие сопровождается накоплением высокоэнергетических токсичных соединений. К ним относятся активные формы кислорода, которые разрушают клеточные мембраны, что и обуславливает их фитотоксичность [4]. Феруловая кислота обнаружена в семенах таких культур как пшеница, овес, рис, кукуруза [5].

Рисунок 3. Механизм действия феруловой кислоты
Рисунок 3. Механизм действия феруловой кислоты

Селективность аллелохимикатов.

Теперь у вас, наверняка, возник закономерный вопрос: “Если феруловая кислота губительна для растений, то как она может в них содержаться?”. Более того, почему гербициды, применяемые фермерами, ведут к гибели сорняков, но не культурных растений? Дело в том, что гербициды делятся на 2 группы:

- сплошного действия, т.е. активные по отношению к широкому ряду растений

- селективные, т.е. оказывающие эффект только на определенные виды растений.

В описанных ранее случаях мы имели дело с селективными аллелохимикатами. Но каковы механизмы подобной избирательности? Согласно книге “How herbicides work” селективность может быть обусловлена несколькими причинами [4]. Основными из них являются 2 нижеприведенных:

1. Действие на специфичные мишени

Выше мы обсуждали, что механизм действия гербицидов связан со связыванием с определенными мишенями. Но оказывается, что мишень может присутствовать в сорняке, но не в культурном растении. В таком случае, ясно, что фитотоксический эффект не будет развиваться у культурного растения.

2. Различие в активности метаболизма

Ряд селективных гербицидов не оказывает губительного действия на культурные растения за счет того, что последние метаболизируют гербициды значительно быстрее, чем сорняки, на которые направлено действие агрохимикатов. Во время метаболизма гербициды претерпевают ряд модификаций, включая конъюгацию с сахарами и аминокислотами. Считается, что эти формы гербицида нетоксичны [4].

К слову о селективности, крупнейшая агрохимическая компания Syngenta предлагает на своем сайте инструмент по подбору избирательного гербицида. Пользователь вводит вид культурного растения и сорняка, от которого хочет избавиться, и получает рекомендацию по гербицидам для данного случая [7].

Как порядочным растениям спастись от гербицидов сплошного действия?

Рассмотрим фитотоксическое действие гербицидов на примере глифосата, т.к. его довольно часто используют в сельском хозяйстве ввиду его эффективности и широкого спектра действия. Его механизм действия основан на связывании с белком EPSP, что ведет к нарушению синтеза ароматических аминокислот. Он может действовать на сорняки как до их всходов, так и после. Вместе с тем, он является неселективным, т.е. может вызывать угнетение роста культурных растений наравне с сорняками. Но если мы все-таки намерены использовать глифосат в силу его эффективности, то как нам быть с его неизбирательностью?

Один из вариантов предложил Джеральд Дилл в своей публикации. В семена сои вводили ген CP4 фермента EPSP из бактерий Agrobacterium tumefaciens, который действует аналогично жизненно важному ферменту EPSP растительных клеток (рис.4), но при этом имеет несколько другую химическую структуру. К чему же это ведет? Глифосат ингибирует “оригинальный” фермент EPSP. При этом фермент бактериального происхождения сохраняет свою функциональность, и жизнедеятельность растений не нарушается. Соя стала первой культурой, устойчивой к глифосату. Генномодифицированные семена сои впервые поступили в продажу в 1996 году под торговой маркой ‘Roundup ready’ компании Monsanto, которую выкупил Bayer в 2018 году [8].

Рисунок 4. Действие глифосата на растение с модицифицированным белком EPSP (CP4)
Рисунок 4. Действие глифосата на растение с модицифицированным белком EPSP (CP4)

Путь гербицида до клетки растения.

Препаративные формы современных гербицидов разнообразны: растворы, эмульсии, смачивающийся порошок, водно-диспергируемые гранулы и прочее [9]. Выбор формы зависит от многих факторов, таких как физико-химические свойства молекул применяемого гербицида, период роста сорняков (довсходовые, послевсходовые), погодных условий и т.п. Подробнее остановимся на водных растворах гербицидов. Нанесение таких растворов осуществляется опрыскиванием. Рассмотрим глифосат. У нас есть цель - сорняк, который растет по центру нашей грядки и мозолит нам глаза. Берём ручной распылитель, заливаем туда раствор глифосата. И вот мы пошли в атаку – первая капля упала на лист. Мы видим, что она не впиталась в лист сию же секунду, а находится на его поверхности, образуя между своей “макушкой” и листом угол, который носит название краевой угол смачивания [10](рис.5) и характеризует степень гидрофобности вещества. Впитыванию капли препятствует восковой слой, находящийся на поверхности листа. Чтобы его преодолеть молекула должна в некой степени быть гидрофобной. В то же время под поверхностными слоями воска находится менее липофильный слой кутина, переплетенный с нитями пектина и внедренного воска (рис. 3). Пектиновые нити гидрофильны и, как полагают, обеспечивают возможность абсорбции гидрофильных гербицидов. Кутикула заканчивается на клеточной стенке подлежащего эпидермиса, для проникновения через который также важна гидрофобность молекулы. Исходя из строения поверхности листа, мы можем сделать вывод, что гербициды должны сохранять баланс между гидрофобными и гидрофильными свойствами. В противном случае, молекула не проникнет в клетку растения.

Рисунок 5. Проникновение гербицидов в растение через лист
Рисунок 5. Проникновение гербицидов в растение через лист

Однако некоторые гербициды способны проникать в растения не только через листья, но еще и через корни, преодолевая довольно непростую преграду на своем пути - почву. Глифосат также относится к их числу. В общем случае, такая способность увеличивает эффективность гербицидов, т.к. они могут действовать на сорняки не только после их всхода, но и до него. Однако и тут есть нюансы. Преодолеть почву не так просто в связи с тем, что ее частицы способны адсорбировать (удерживать) наше вещество, ограждая его от растения. Степень адсорбции вещества почвой характеризуется значением коэффициента распределения органического углерода – Koc, [г/мл]. Изменение степени адсорбции гербицида почвой прямо пропорционально изменению Koc, т.е. чем больше Koc, тем больше степень адсорбции гербицида почвой и тем больше вероятность того, что бОльшая часть молекул гербицида не достигнет корней сорняка. Кос зависит от ряда параметров почвы, таких как влажность и pH.

А при чем тут ИИ?

Бурное внедрение ИИ в науки о жизни не обошло стороной сельское хозяйство. В частности, это актуально для борьбы с сорняками. Так, M.Mehdizadeh с коллегами выделяют следующие стратегии применения ИИ в контексте этой задачи [11]:

1) Прогностическое моделирование роста сорняков

Используя ML-модели для прогнозирования роста сорняков, фермеры могут более эффективно планировать применение гербицидов;

2) Определение видов сорняков

Модели распознавания изображений позволяют автоматизировать процесс идентификации видов сорняков, необходимый для корректного подбора гербицида;

3) Системы поддержки принятия решений фермеров на основе машинного обучения

Подобные системы учитывают такие факторы, как погодные условия, свойства почвы, исторические данные, чтобы рекомендовать наиболее подходящий гербицид, дозу и время внесения;

4) Робототехника

Роботизированные системы используют алгоритмы машинного обучения для навигации по полям, определения сорняков и места для внесения гербицидов.

Рисунок 6. Применение ИИ для борьбы с сорняками
Рисунок 6. Применение ИИ для борьбы с сорняками

Перечисленные пункты – только часть возможного применения ИИ для борьбы с сорняками (рис.6). Как известно, благодаря технологиям ИИ возможно осуществлять предсказания различных свойств молекул. Возвращаясь к нашей теме, могут быть построены модели машинного обучения для прогнозирования фитотоксичности. Подобный инструмент необходим для разработки новых гербицидов, т.к. позволяет идентифицировать наиболее перспективные соединения в больших массивах химических данных. Однако остается размытым понятие фитотоксичности: как ее определяют экспериментально, в чем она измеряется? Принципиально понимать ответ на этот вопрос, так как он связан с обучающими данными для нашей модели. В действительности, есть по меньшей мере два основных подхода для формализации фитотоксичности:

1) Определение активности по отношению к белку-мишени

Как мы обсуждали ранее, механизм действия гербицидов связан с действием на определенные мишени (как правило, белки). Поэтому один из подходов определения фитотоксического эффекта связан с измерением активности гербицида по отношению к белку-мишени. Так, Симона Фунар-Тимофей c коллегами в своей статье исследовали степень ингибирования белка D1, участвующего в фотосинтезе. В качестве параметра фитотоксичности автор использовал показатель IC50 (Inhibition Concentration 50), который соответствует концентрации молекулы (гербицида) необходимой для замедления работы белка на 50%. Чем меньше значение IC50, тем активнее молекула. Для предсказания данного показателя автор использовал метод множественной линейной регрессии [12].

2) Определение фенотипических проявлений фитотоксичности

В то же время, очевидно, что вредоносное действие гербицида может быть оценено визуально. Однако не совсем понятно, как это однозначно формализовать. Известен целый ряд подходов. Так, в своей статье Лунфэн Ван с коллегами измеряли зависимость изменения массы растения от исследуемого гербицида. Чем выраженнее фитотоксичность гербицида, тем меньше масса, которой достигают растения к заданному моменту времени (рис.7). Также исследователи отслеживали изменения количества хлорофилла в растениях и площади листа [13].

Yuki Nakayama с коллегами оценивали фитотоксический эффект гербицидов по шкале от 0 до 100. Для предсказания того, обладают ли какие-либо химические вещества гербицидной активностью авторы применяли такие методы классификации как линейные и нелинейные методы опорных векторов (SVM) и случайного леса (Random forest) [14].

Рисунок 7. Фенотипическая оценка фитотоксичности
Рисунок 7. Фенотипическая оценка фитотоксичности

Всё больше крупных агрохимических компаний ищут специалистов в области машинного обучения и Data Science. Так, упоминавшаяся ранее Syngenta регулярно публикует вакансии на позицию хемоинформатика [15]. Кроме того, Syngenta создала систему ИИ ‘Cropwise AI’, которая способна предсказывать устойчивость сорняков к гербицидам для выбора более подходящего и эффективного агрохимиката [16].

Компания Bayer, в свою очередь, разработала систему искусственного интеллекта Cropkey, которая ускоряет поиск новых пестицидов. Первым продуктом, разработанным с помощью системы CropKey, стал послевсходовый гербицид Incafolin. Планируется его выход на рынок Бразилии в 2028 году [17].

Заключение.

Стоимость разработки нового гербицида по данным “Crop Life International” составляет порядка 300 млн $ [18]. То есть для открытия всего лишь одного нового гербицида требуется инвестировать сумму сопоставимую с годовым доходом республики Алтай (население > 200 тыс.). Такая высокая цена обусловлена необходимостью проведения большого количества экспериментов в ходе разработки. В то же время, путем предсказания фитотоксичности и других свойств молекул с помощью технологий ИИ мы можем значительно сократить время и материальные затраты на этой стадии. При этом важно понимать, что получение новых гербицидов — это не только коммерческий интерес агрохимических компаний. Эффективные гербициды — залог благополучия сельского хозяйства, а, в конце концов, и всех потребителей сельхозпродукции.

В последнее время активно обсуждается проблема резистентности сорняков к гербицидам [19]. Это значит, что с течением времени ряд сорных растений приспосабливается к токсичным для них веществам, и фермеры лишаются инструментов борьбы с ними. Быстрый и эффективный поиск новых гербицидов является краеугольным камнем в контексте данной проблемой, а инструменты на основе ИИ - многообещающим решением.

Таким образом, роль применения ИИ в современной агрохимии выходит на передовые позиции, что способствует повышению устойчивости сельского хозяйства к изменениям в экосистемах и обеспечивает продовольственную безопасность населения.

Список литературы

1. Molisch H. “The Influence of One Plant on Another: Allelopathy”, 2001 (1937)

2. José L. Hierro,Ragan M. Callaway“The Ecological Importance of Allelopathy”,2021 https://doi.org/10.1146/annurev-ecolsys-051120-030619

3. Sobia Anwar, Saadia Naseem, Zahid Ali «Biochemical analysis, photosynthetic gene (psbA) down–regulation, and in silico receptor prediction in weeds in response to exogenous application of phenolic acids and their analogs», 2023 https://doi.org/10.1371/journal.pone.0277146

4. How herbicides work : biology to application : Hall, Linda : Free Download, Borrow, and Streaming : Internet Archive

5. Hüseyin BOZ «Ferulic acid in cereals – a review», 2015 DOI:10.17221/401/2014-CJFS https://www.semanticscholar.org/paper/Ferulic-Acid-in-Cereals-a-Review-Boz/74cdc0ad3811d95b92c1ecb55ddea392de95ba59

6. Carvalho et al. Herbicide selectivity by differential metabolism: considerations for reducing crop damages, 2009 https://doi.org/10.1590/S0103-90162009000100020

7. https://www.syngenta.ru/onlajn-kalkulyator-reshenie-po-sornyakam

8. Gerald M Dill «Glyphosate-resistant crops: history, status and future», 2005 https://pubmed.ncbi.nlm.nih.gov/15662720/ DOI: 10.1002/ps.1008

9. Hazra, D.K. "Conventional and futuristic smart herbicide formulations for environment friendly weed management: a review.", 2018 https://www.researchgate.net/publication/327931247_Conventional_and_futuristic_smart_herbicide_formulations_for_environment_friendly_weed_management_A_review

10. Jingjing Wei et al. «Wettability on plant leaf surfaces and its effect on pesticide efficiency», 2020 https://www.researchgate.net/publication/340293700_Wettability_on_plant_leaf_surfaces_and_its_effect_on_pesticide_efficiency DOI:10.33440/j.ijpaa.20200301.62

11. Daniel J. Bloomer et al. “Robots and shocks: emerging non-herbicide weed controloptions for vegetable and arable cropping”, 2024 https://www.tandfonline.com/doi/full/10.1080/00288233.2023.2252769

12. Simona Funar-Timofei et al. “Combined molecular docking and QSAR study of fused heterocyclic herbicide inhibitors of D1 protein in photosystem II of plants”, 2017 https://link.springer.com/article/10.1007/s11030-017-9735-x

13. Longfeng Wang et al. ‘Study on phytotoxicity evaluation and physiological properties of nicosulfuron on sugar beet (Beta vulgaris L.)’, 2022 https://doi.org/10.3389/fpls.2022.998867

14. Yuki Nakayama et al. “Molecular Design of Novel Herbicide and Insecticide SeedCompounds with Machine Learning”, 2024 Molecular Design of Novel Herbicide and Insecticide Seed Compounds with Machine Learning | ACS Omega

15. https://jobs.syngenta.com/job/postdoctoral-fellow-m-f-d-cheminformatics-data-scientist-2-years-in-ch-stein-jid-10398

16. Syngenta Group adds Cutting-Edge Generative Artificial Intelligence (GenAI) to Cropwise | Syngenta Group

17. Bayer (CropKey) support regenerate agriculture! A number of new pesticide compounds in the R&D pipeline are coming to market!

18. Time-and-Cost-To-Market-CP-2024.pdf - Yandex Documents

19. Jussaume, R., & Ervin, D. (2016). Understanding Weed Resistance as a Wicked Problem to Improve Weed Management Decisions. Weed Science, 64, 559 - 569. https://doi.org/10.1614/WS-D-15-00131.1.

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  • 07.09.23 16:24 CherryTeam

    Cherry Team atlyginimų skaičiavimo programa yra labai naudingas įrankis įmonėms, kai reikia efektyviai valdyti ir skaičiuoti darbuotojų atlyginimus. Ši programinė įranga, turinti išsamias funkcijas ir patogią naudotojo sąsają, suteikia daug privalumų, kurie padeda supaprastinti darbo užmokesčio skaičiavimo procesus ir pagerinti finansų valdymą. Štai keletas pagrindinių priežasčių, kodėl Cherry Team atlyginimų skaičiavimo programa yra naudinga įmonėms: Automatizuoti ir tikslūs skaičiavimai: Atlyginimų skaičiavimai rankiniu būdu gali būti klaidingi ir reikalauti daug laiko. Programinė įranga Cherry Team automatizuoja visą atlyginimų skaičiavimo procesą, todėl nebereikia atlikti skaičiavimų rankiniu būdu ir sumažėja klaidų rizika. Tiksliai apskaičiuodama atlyginimus, įskaitant tokius veiksnius, kaip pagrindinis atlyginimas, viršvalandžiai, premijos, išskaitos ir mokesčiai, programa užtikrina tikslius ir be klaidų darbo užmokesčio skaičiavimo rezultatus. Sutaupoma laiko ir išlaidų: Darbo užmokesčio valdymas gali būti daug darbo jėgos reikalaujanti užduotis, reikalaujanti daug laiko ir išteklių. Programa Cherry Team supaprastina ir pagreitina darbo užmokesčio skaičiavimo procesą, nes automatizuoja skaičiavimus, generuoja darbo užmokesčio žiniaraščius ir tvarko išskaičiuojamus mokesčius. Šis automatizavimas padeda įmonėms sutaupyti daug laiko ir pastangų, todėl žmogiškųjų išteklių ir finansų komandos gali sutelkti dėmesį į strategiškai svarbesnę veiklą. Be to, racionalizuodamos darbo užmokesčio operacijas, įmonės gali sumažinti administracines išlaidas, susijusias su rankiniu darbo užmokesčio tvarkymu. Mokesčių ir darbo teisės aktų laikymasis: Įmonėms labai svarbu laikytis mokesčių ir darbo teisės aktų, kad išvengtų baudų ir teisinių problemų. Programinė įranga Cherry Team seka besikeičiančius mokesčių įstatymus ir darbo reglamentus, užtikrindama tikslius skaičiavimus ir teisinių reikalavimų laikymąsi. Programa gali dirbti su sudėtingais mokesčių scenarijais, pavyzdžiui, keliomis mokesčių grupėmis ir įvairių rūšių atskaitymais, todėl užtikrina atitiktį reikalavimams ir kartu sumažina klaidų riziką. Ataskaitų rengimas ir analizė: Programa Cherry Team siūlo patikimas ataskaitų teikimo ir analizės galimybes, suteikiančias įmonėms vertingų įžvalgų apie darbo užmokesčio duomenis. Ji gali generuoti ataskaitas apie įvairius aspektus, pavyzdžiui, darbo užmokesčio paskirstymą, išskaičiuojamus mokesčius ir darbo sąnaudas. Šios ataskaitos leidžia įmonėms analizuoti darbo užmokesčio tendencijas, nustatyti tobulintinas sritis ir priimti pagrįstus finansinius sprendimus. Pasinaudodamos duomenimis pagrįstomis įžvalgomis, įmonės gali optimizuoti savo darbo užmokesčio strategijas ir veiksmingai kontroliuoti išlaidas. Integracija su kitomis sistemomis: Cherry Team programinė įranga dažnai sklandžiai integruojama su kitomis personalo ir apskaitos sistemomis. Tokia integracija leidžia automatiškai perkelti atitinkamus duomenis, pavyzdžiui, informaciją apie darbuotojus ir finansinius įrašus, todėl nebereikia dubliuoti duomenų. Supaprastintas duomenų srautas tarp sistemų padidina bendrą efektyvumą ir sumažina duomenų klaidų ar neatitikimų riziką. Cherry Team atlyginimų apskaičiavimo programa įmonėms teikia didelę naudą - automatiniai ir tikslūs skaičiavimai, laiko ir sąnaudų taupymas, atitiktis mokesčių ir darbo teisės aktų reikalavimams, ataskaitų teikimo ir analizės galimybės bei integracija su kitomis sistemomis. Naudodamos šią programinę įrangą įmonės gali supaprastinti darbo užmokesčio skaičiavimo procesus, užtikrinti tikslumą ir atitiktį reikalavimams, padidinti darbuotojų pasitenkinimą ir gauti vertingų įžvalgų apie savo finansinius duomenis. Programa Cherry Team pasirodo esanti nepakeičiamas įrankis įmonėms, siekiančioms efektyviai ir veiksmingai valdyti darbo užmokestį. https://cherryteam.lt/lt/

  • 08.10.23 01:30 davec8080

    The "Shibarium for this confirmed rug pull is a BEP-20 project not related at all to Shibarium, SHIB, BONE or LEASH. The Plot Thickens. Someone posted the actual transactions!!!! https://bscscan.com/tx/0xa846ea0367c89c3f0bbfcc221cceea4c90d8f56ead2eb479d4cee41c75e02c97 It seems the article is true!!!! And it's also FUD. Let me explain. Check this link: https://bscscan.com/token/0x5a752c9fe3520522ea88f37a41c3ddd97c022c2f So there really is a "Shibarium" token. And somebody did a rug pull with it. CONFIRMED. But the "Shibarium" token for this confirmed rug pull is a BEP-20 project not related at all to Shibarium, SHIB, BONE or LEASH.

  • 24.06.24 04:31 tashandiarisha

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  • 26.06.24 18:46 Jacobethannn098

    LEGAL RECOUP FOR CRYPTO THEFT BY ADRIAN LAMO HACKER

  • 26.06.24 18:46 Jacobethannn098

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  • 04.07.24 04:49 ZionNaomi

    For over twenty years, I've dedicated myself to the dynamic world of marketing, constantly seeking innovative strategies to elevate brand visibility in an ever-evolving landscape. So when the meteoric rise of Bitcoin captured my attention as a potential avenue for investment diversification, I seized the opportunity, allocating $20,000 to the digital currency. Witnessing my investment burgeon to an impressive $70,000 over time instilled in me a sense of financial promise and stability.However, amidst the euphoria of financial growth, a sudden and unforeseen oversight brought me crashing back to reality during a critical business trip—I had misplaced my hardware wallet. The realization that I had lost access to the cornerstone of my financial security struck me with profound dismay. Desperate for a solution, I turned to the expertise of Daniel Meuli Web Recovery.Their response was swift . With meticulous precision, they embarked on the intricate process of retracing the elusive path of my lost funds. Through their unwavering dedication, they managed to recover a substantial portion of my investment, offering a glimmer of hope amidst the shadows of uncertainty. The support provided by Daniel Meuli Web Recovery extended beyond mere financial restitution. Recognizing the imperative of fortifying against future vulnerabilities, they generously shared invaluable insights on securing digital assets. Their guidance encompassed crucial aspects such as implementing hardware wallet backups and fortifying security protocols, equipping me with recovered funds and newfound knowledge to navigate the digital landscape securely.In retrospect, this experience served as a poignant reminder of the critical importance of diligence and preparedness in safeguarding one's assets. Thanks to the expertise and unwavering support extended by Daniel Meuli Web Recovery, I emerged from the ordeal with renewed resilience and vigilance. Empowered by their guidance and fortified by enhanced security measures, I now approach the future with unwavering confidence.The heights of financial promise to the depths of loss and back again has been a humbling one, underscoring the volatility and unpredictability inherent in the digital realm. Yet, through adversity, I have emerged stronger, armed with a newfound appreciation for the importance of diligence, preparedness, and the invaluable support of experts like Daniel Meuli Web Recovery.As I persist in traversing the digital landscape, I do so with a judicious blend of vigilance and fortitude, cognizant that with adequate safeguards and the backing of reliable confidants, I possess the fortitude to withstand any adversity that may arise. For this, I remain eternally appreciative. Email Danielmeuliweberecovery @ email . c om WhatsApp + 393 512 013 528

  • 13.07.24 21:13 michaelharrell825

    In 2020, amidst the economic fallout of the pandemic, I found myself unexpectedly unemployed and turned to Forex trading in hopes of stabilizing my finances. Like many, I was drawn in by the promise of quick returns offered by various Forex robots, signals, and trading advisers. However, most of these products turned out to be disappointing, with claims that were far from reality. Looking back, I realize I should have been more cautious, but the allure of financial security clouded my judgment during those uncertain times. Amidst these disappointments, Profit Forex emerged as a standout. Not only did they provide reliable service, but they also delivered tangible results—a rarity in an industry often plagued by exaggerated claims. The positive reviews from other users validated my own experience, highlighting their commitment to delivering genuine outcomes and emphasizing sound financial practices. My journey with Profit Forex led to a net profit of $11,500, a significant achievement given the challenges I faced. However, my optimism was short-lived when I encountered obstacles trying to withdraw funds from my trading account. Despite repeated attempts, I found myself unable to access my money, leaving me frustrated and uncertain about my financial future. Fortunately, my fortunes changed when I discovered PRO WIZARD GIlBERT RECOVERY. Their reputation for recovering funds from fraudulent schemes gave me hope in reclaiming what was rightfully mine. With a mixture of desperation and cautious optimism, I reached out to them for assistance. PRO WIZARD GIlBERT RECOVERY impressed me from the start with their professionalism and deep understanding of financial disputes. They took a methodical approach, using advanced techniques to track down the scammers responsible for withholding my funds. Throughout the process, their communication was clear and reassuring, providing much-needed support during a stressful period. Thanks to PRO WIZARD GIlBERT RECOVERY's expertise and unwavering dedication, I finally achieved a resolution to my ordeal. They successfully traced and retrieved my funds, restoring a sense of justice and relief. Their intervention not only recovered my money but also renewed my faith in ethical financial services. Reflecting on my experience, I've learned invaluable lessons about the importance of due diligence and discernment in navigating the Forex market. While setbacks are inevitable, partnering with reputable recovery specialists like PRO WIZARD GIlBERT RECOVERY can make a profound difference. Their integrity and effectiveness have left an indelible mark on me, guiding my future decisions and reinforcing the value of trustworthy partnerships in achieving financial goals. I wholeheartedly recommend PRO WIZARD GIlBERT RECOVERY to anyone grappling with financial fraud or disputes. Their expertise and commitment to client satisfaction are unparalleled, offering a beacon of hope in challenging times. Thank you, PRO WIZARD GIlBERT RECOVERY, for your invaluable assistance in reclaiming what was rightfully mine. Your service not only recovered my funds but also restored my confidence in navigating the complexities of financial markets with greater caution and awareness. Email: prowizardgilbertrecovery(@)engineer.com Homepage: https://prowizardgilbertrecovery.xyz WhatsApp: +1 (516) 347‑9592

  • 17.07.24 02:26 thompsonrickey

    In the vast and often treacherous realm of online investments, I was entangled in a web of deceit that cost me nearly  $45,000. It all started innocuously enough with an enticing Instagram profile promising lucrative returns through cryptocurrency investment. Initially, everything seemed promising—communications were smooth, and assurances were plentiful. However, as time passed, my optimism turned to suspicion. Withdrawal requests were met with delays and excuses. The once-responsive "investor" vanished into thin air, leaving me stranded with dwindling hopes and a sinking feeling in my gut. It became painfully clear that I had been duped by a sophisticated scheme designed to exploit trust and naivety. Desperate to recover my funds, I turned to online forums where I discovered numerous testimonials advocating for Muyern Trust Hacker. With nothing to lose, I contacted them, recounting my ordeal with a mixture of skepticism and hope. Their swift response and professional demeanor immediately reassured me that I had found a lifeline amidst the chaos. Muyern Trust Hacker wasted no time in taking action. They meticulously gathered evidence, navigated legal complexities, and deployed their expertise to expedite recovery. In what felt like a whirlwind of activity, although the passage of time was a blur amidst my anxiety, they achieved the seemingly impossible—my stolen funds were returned. The relief I felt was overwhelming. Muyern Trust Hacker not only restored my financial losses but also restored my faith in justice. Their commitment to integrity and their relentless pursuit of resolution were nothing short of remarkable. They proved themselves as recovery specialists and guardians against digital fraud, offering hope to victims like me who had been ensnared by deception. My gratitude knows no bounds for Muyern Trust Hacker. Reach them at muyerntrusted @ m a i l - m e . c o m AND Tele gram @ muyerntrusthackertech

  • 18.07.24 20:13 austinagastya

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  • 27.08.24 12:50 James889900

    All you need is to hire an expert to help you accomplish that. If there’s any need to spy on your partner’s phone. From my experience I lacked evidence to confront my husband on my suspicion on his infidelity, until I came across ETHICALAHCKERS which many commend him of assisting them in their spying mission. So I contacted him and he provided me with access into his phone to view all text messages, call logs, WhatsApp messages and even her location. This evidence helped me move him off my life . I recommend you consult ETHICALHACKERS009 @ gmail.com OR CALL/TEXT ‪+1(716) 318-5536 or whatsapp +14106350697 if you need access to your partner’s phone

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  • 02.09.24 20:24 [email protected]

    If You Need Hacker To Recover Your Bitcoin Contact Paradox Recovery Wizard Paradox Recovery Wizard successfully recovered $123,000 worth of Bitcoin for my husband, which he had lost due to a security breach. The process was efficient and secure, with their expert team guiding us through each step. They were able to trace and retrieve the lost cryptocurrency, restoring our peace of mind and financial stability. Their professionalism and expertise were instrumental in recovering our assets, and we are incredibly grateful for their service. Email: support@ paradoxrecoverywizard.com Email: paradox_recovery @cyberservices.com Wep: https://paradoxrecoverywizard.com/ WhatsApp: +39 351 222 3051.

  • 06.09.24 01:35 Celinagarcia

    HOW TO RECOVER MONEY LOST IN BITCOIN/USDT TRADING OR TO CRYPTO INVESTMENT !! Hi all, friends and families. I am writing From Alberton Canada. Last year I tried to invest in cryptocurrency trading in 2023, but lost a significant amount of money to scammers. I was cheated of my money, but thank God, I was referred to Hack Recovery Wizard they are among the best bitcoin recovery specialists on the planet. they helped me get every penny I lost to the scammers back to me with their forensic techniques. and I would like to take this opportunity to advise everyone to avoid making cryptocurrency investments online. If you ​​​​​​have already lost money on forex, cryptocurrency or Ponzi schemes, please contact [email protected] or WhatsApp: +1 (757) 237–1724 at once they can help you get back the crypto you lost to scammers. BEST WISHES. Celina Garcia.

  • 06.09.24 01:44 Celinagarcia

    HOW TO RECOVER MONEY LOST IN BITCOIN/USDT TRADING OR TO CRYPTO INVESTMENT !! Hi all, friends and families. I am writing From Alberton Canada. Last year I tried to invest in cryptocurrency trading in 2023, but lost a significant amount of money to scammers. I was cheated of my money, but thank God, I was referred to Hack Recovery Wizard they are among the best bitcoin recovery specialists on the planet. they helped me get every penny I lost to the scammers back to me with their forensic techniques. and I would like to take this opportunity to advise everyone to avoid making cryptocurrency investments online. If you ​​​​​​have already lost money on forex, cryptocurrency or Ponzi schemes, please contact [email protected] or WhatsApp: +1 (757) 237–1724 at once they can help you get back the crypto you lost to scammers. BEST WISHES. Celina Garcia.

  • 16.09.24 00:10 marcusaustin

    Bitcoin Recovery Services: Restoring Lost Cryptocurrency If you've lost access to your cryptocurrency and unable to make a withdrawal, I highly recommend iBolt Cyber Hacker Bitcoin Recovery Services. Their team is skilled, professional, and efficient in recovering lost Bitcoin. They provide clear communication, maintain high security standards, and work quickly to resolve issues. Facing the stress of lost cryptocurrency, iBolt Cyber Hacker is a trusted service that will help you regain access to your funds securely and reliably. Highly recommended! Email: S u p p o r t @ ibolt cyber hack . com Cont/Whtp + 3. .9 .3. .5..0. .9. 2. 9. .0 .3. 1 .8. Website: h t t p s : / / ibolt cyber hack . com /

  • 16.09.24 00:11 marcusaustin

    Bitcoin Recovery Services: Restoring Lost Cryptocurrency If you've lost access to your cryptocurrency and unable to make a withdrawal, I highly recommend iBolt Cyber Hacker Bitcoin Recovery Services. Their team is skilled, professional, and efficient in recovering lost Bitcoin. They provide clear communication, maintain high security standards, and work quickly to resolve issues. Facing the stress of lost cryptocurrency, iBolt Cyber Hacker is a trusted service that will help you regain access to your funds securely and reliably. Highly recommended! Email: S u p p o r t @ ibolt cyber hack . com Cont/Whtp + 3. .9 .3. .5..0. .9. 2. 9. .0 .3. 1 .8. Website: h t t p s : / / ibolt cyber hack . com /

  • 23.09.24 18:56 matthewshimself

    At first, I was admittedly skeptical about Worldcoin (ref: https://worldcoin.org/blog/worldcoin/this-is-worldcoin-video-explainer-series), particularly around the use of biometric data and the WLD token as a reward mechanism for it. However, after following the project closer, I’ve come to appreciate the broader vision and see the value in the underlying tech behind it. The concept of Proof of Personhood (ref: https://worldcoin.org/blog/worldcoin/proof-of-personhood-what-it-is-why-its-needed) has definitely caught my attention, and does seem like a crucial step towards tackling growing issues like bots, deepfakes, and identity fraud. Sam Altman’s vision is nothing short of ambitious, but I do think he & Alex Blania have the chops to realize it as mainstay in the global economy.

  • 01.10.24 14:54 Sinewclaudia

    I lost about $876k few months ago trading on a fake binary option investment websites. I didn't knew they were fake until I tried to withdraw. Immediately, I realized these guys were fake. I contacted Sinew Claudia world recovery, my friend who has such experience before and was able to recover them, recommended me to contact them. I'm a living testimony of a successful recovery now. You can contact the legitimate recovery company below for help and assistance. [email protected] [email protected] WhatsApp: 6262645164

  • 02.10.24 22:27 Emily Hunter

    Can those who have fallen victim to fraud get their money back? Yes, you might be able to get back what was taken from you if you fell prey to a fraud from an unregulated investing platform or any other scam, but only if you report it to the relevant authorities. With the right plan and supporting documentation, you can get back what you've lost. Most likely, the individuals in control of these unregulated platforms would attempt to convince you that what happened to your money was a sad accident when, in fact, it was a highly skilled heist. You should be aware that there are resources out there to help you if you or someone you know has experienced one of these circumstances. Do a search using (deftrecoup (.) c o m). Do not let the perpetrators of this hoaxes get away with ruining you mentally and financially.

  • 18.10.24 09:34 freidatollerud

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  • 31.10.24 00:13 ytre89

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  • 12.11.24 00:50 TERESA

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  • 21.11.24 04:14 ronaldandre617

    Being a parent is great until your toddler figures out how to use your devices. One afternoon, I left my phone unattended for just a few minutes rookie mistake of the century. I thought I’d take a quick break, but little did I know that my curious little genius was about to embark on a digital adventure. By the time I came back, I was greeted by two shocking revelations: my toddler had somehow managed to buy a $5 dinosaur toy online and, even more alarmingly, had locked me out of my cryptocurrency wallet holding a hefty $75,000. Yes, you heard that right a dinosaur toy was the least of my worries! At first, I laughed it off. I mean, what toddler doesn’t have a penchant for expensive toys? But then reality set in. I stared at my phone in disbelief, desperately trying to guess whatever random string of gibberish my toddler had typed as a new password. Was it “dinosaur”? Or perhaps “sippy cup”? I felt like I was in a bizarre game of Password Gone Wrong. Every attempt led to failure, and soon the laughter faded, replaced by sheer panic. I was in way over my head, and my heart raced as the countdown of time ticked away. That’s when I decided to take action and turned to Digital Tech Guard Recovery, hoping they could solve the mystery that was my toddler’s handiwork. I explained my predicament, half-expecting them to chuckle at my misfortune, but they were incredibly professional and empathetic. Their confidence put me at ease, and I knew I was in good hands. Contact With WhatsApp: +1 (443) 859 - 2886  Email digital tech guard . com  Telegram: digital tech guard recovery . com  website link :: https : // digital tech guard . com Their team took on the challenge like pros, employing their advanced techniques to unlock my wallet with a level of skill I can only describe as magical. As I paced around, anxiously waiting for updates, I imagined my toddler inadvertently locking away my life savings forever. But lo and behold, it didn’t take long for Digital Tech Guard Recovery to work their magic. Not only did they recover the $75,000, but they also gave me invaluable tips on securing my wallet better like not leaving it accessible to tiny fingers! Who knew parenting could lead to such dramatic situations? Crisis averted, and I learned my lesson: always keep my devices out of reach of little explorers. If you ever find yourself in a similar predicament whether it’s tech-savvy toddlers or other digital disasters don’t hesitate to reach out to Digital Tech Guard Recovery. They saved my funds and my sanity, proving that no challenge is too great, even when it involves a toddler’s mischievous fingers!

  • 21.11.24 08:02 Emily Hunter

    If I hadn't found a review online and filed a complaint via email to support@deftrecoup. com , the people behind this unregulated scheme would have gotten away with leaving me in financial ruins. It was truly the most difficult period of my life.

  • 22.11.24 04:41 [email protected]

    I never could have imagined the nightmare of losing access to my digital wallet. All of my cryptocurrency holdings were abruptly imprisoned, inaccessible, and appeared to be lost forever following a catastrophic hardware breakdown. Years of meticulous investment and careful saving were reduced to nothing more than strings of code that I could no longer control, and I could feel the dread and sorrow that swept through me at that very instant. Thankfully, during my worst moment, I came into (TRUST GEEKS HACK EXPERT), a professional service devoted to recovering lost or inaccessible digital data. With optimism, I went out to their team of skilled technologists, laying bare the full nature of my issue. What followed was a laborious, multi-step process that required an almost surgical level of digital forensics and Bitcoin skill. In order to create a thorough profile of my wallet's contents and activities, the (TRUST GEEKS HACK EXPERT) team first thoroughly examined the transaction history and metadata connected to it. Next, they implemented a series of advanced recovery techniques, using cutting-edge software tools to bypass the access barriers that had left me locked out. The entire process was shrouded in secrecy and discretion, with the (TRUST GEEKS HACK EXPERT) team working tirelessly to protect the confidentiality of my sensitive financial information. After what felt like an eternity of nervous anticipation, the day finally arrived when I received the triumphant notification – my wallet had been successfully restored, and all of my precious digital assets had been returned to my control. The sense of relief was indescribable, as I could finally breathe easy knowing that the fruits of my financial discipline had been safeguarded. While the experience of losing access to my wallet was undoubtedly traumatic, (TRUST GEEKS HACK EXPERT) intervention allowed me to emerge from the ordeal with my cryptocurrency holdings intact, and a renewed appreciation for the importance of proactive digital asset management. You can contact Them through EMAIL: [email protected] - TELEGRAM: TRUSTGEEKSHACKEXPERT

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  • 22.11.24 23:43 teresaborja

    all thanks to Tech Cyber Force Recovery expert assistance. As a novice in cryptocurrency, I had been carefully accumulating a modest amount of Bitcoin, meticulously safeguarding my digital wallet and private keys. However, as the adage goes, the best-laid plans can often go awry, and that's precisely what happened to me. Due to a series of technical mishaps and human errors, I found myself locked out of my Bitcoin wallet, unable to access the fruits of my digital labors. Panic set in as I frantically searched for a solution, scouring the internet for any glimmer of hope. That's when I stumbled upon the Tech Cyber Force Recovery team, a group of seasoned cryptocurrency specialists who had built a reputation for their ability to recover lost or inaccessible digital assets. Skeptical at first, I reached out, desperate for a miracle. To my utter amazement, the Tech Cyber Force Recovery experts quickly assessed my situation and devised a meticulous plan of attack. Through their deep technical knowledge, unwavering determination, and a keen eye for detail, they were able to navigate the complex labyrinth of blockchain technology, ultimately recovering my entire Bitcoin portfolio. What had once seemed like a hopeless endeavor was now a reality, and I found myself once again in possession of my digital wealth, all thanks to the incredible efforts of the Tech Cyber Force Recovery team. This experience has not only restored my faith in the cryptocurrency ecosystem. Still, it has also instilled in me a profound appreciation for the critical role that expert recovery services can play in safeguarding one's digital assets.   ENAIL < Tech cybers force recovery @ cyber services. com >   WEBSITE < ht tps : // tech cyber force recovery. info  >   TEXT < +1. 561. 726. 3697 >

  • 24.11.24 02:21 [email protected]

    I never could have imagined the nightmare of losing access to my digital wallet. All of my cryptocurrency holdings were abruptly imprisoned, inaccessible, and appeared to be lost forever following a catastrophic hardware breakdown. Years of meticulous investment and careful saving were reduced to nothing more than strings of code that I could no longer control, and I could feel the dread and sorrow that swept through me at that very instant. Thankfully, during my worst moment, I came into (TRUST GEEKS HACK EXPERT), a professional service devoted to recovering lost or inaccessible digital data. With optimism, I went out to their team of skilled technologists, laying bare the full nature of my issue. What followed was a laborious, multi-step process that required an almost surgical level of digital forensics and Bitcoin skill. In order to create a thorough profile of my wallet's contents and activities, the (TRUST GEEKS HACK EXPERT) team first thoroughly examined the transaction history and metadata connected to it. Next, they implemented a series of advanced recovery techniques, using cutting-edge software tools to bypass the access barriers that had left me locked out. The entire process was shrouded in secrecy and discretion, with the (TRUST GEEKS HACK EXPERT) team working tirelessly to protect the confidentiality of my sensitive financial information. After what felt like an eternity of nervous anticipation, the day finally arrived when I received the triumphant notification – my wallet had been successfully restored, and all of my precious digital assets had been returned to my control. The sense of relief was indescribable, as I could finally breathe easy knowing that the fruits of my financial discipline had been safeguarded. While the experience of losing access to my wallet was undoubtedly traumatic, (TRUST GEEKS HACK EXPERT) intervention allowed me to emerge from the ordeal with my cryptocurrency holdings intact, and a renewed appreciation for the importance of proactive digital asset management. You can contact Them through EMAIL: [email protected] - TELEGRAM: TRUSTGEEKSHACKEXPERT

  • 25.11.24 02:19 briankennedy

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  • 25.11.24 02:20 briankennedy

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  • 26.11.24 21:59 [email protected]

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  • 26.11.24 23:12 rickrobinson8

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  • 26.11.24 23:12 rickrobinson8

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  • 27.11.24 00:39 [email protected]

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  • 27.11.24 09:10 Michal Novotny

    The biggest issue with cryptocurrency is that it is unregulated, wh ich is why different people can come up with different fake stories all the time, and it is unfortunate that platforms like Facebook and others only care about the money they make from them through ads. I saw an ad on Facebook for Cointiger and fell into the scam, losing over $30,000. I reported it to Facebook, but they did nothing until I discovered deftrecoup . c o m from a crypto community; they retrieved approximately 95% of the total amount I lost.

  • 01.12.24 17:21 KollanderMurdasanu

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  • 02.12.24 23:02 ytre89

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  • 04.12.24 22:24 andreygagloev

    When I first heard about Bitcoin back in 2018, I was skeptical. The idea of a decentralized, digital currency seemed too good to be true. But I was intrigued as I learned more about the technology behind it and its potential. I started small, investing just a few hundred dollars, dipping my toes into the cryptocurrency waters. At first, it was exhilarating to watch the value of my investment grow exponentially. I felt like I was part of the future, an early adopter of this revolutionary new asset. But that euphoria was short-lived. One day, I logged into my digital wallet only to find it empty - my Bitcoin had vanished without a trace. It turned out that the online exchange I had trusted had been hacked, and my funds were stolen. I was devastated, both financially and emotionally. All the potential I had seen in Bitcoin was tainted by the harsh reality that with decentralization came a lack of regulation and oversight. My hard-earned money was gone, lost to the ether of the digital world. This experience taught me a painful lesson about the price of trust in the uncharted territory of cryptocurrency. While the technology holds incredible promise, the risks can be catastrophic if you don't approach it with extreme caution. My Bitcoin investment gamble had failed, and I was left to pick up the pieces, wiser but poorer for having placed my faith in the wrong hands. My sincere appreciation goes to MUYERN TRUST HACKER. You are my hero in recovering my lost funds. Send a direct m a i l ( muyerntrusted ( @ ) mail-me ( . )c o m ) or message on whats app : + 1 ( 4-4-0 ) ( 3 -3 -5 ) ( 0-2-0-5 )

  • 12.12.24 00:35 amandagregory

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  • 12.12.24 00:35 amandagregory

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  • 19.12.24 17:07 rebeccabenjamin

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