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ИИ помог обойти защиту резистентных бактерий: открыт новый класс перспективных антибиотиков

По прогнозам к 2050 году смертность от инфекционных и бактериальных заболеваний, не поддающихся лечению в связи с устойчивостью возбудителей к антибактериальным препаратам, составит 10 млн человек в год и выйдет на одно из лидирующих мест наряду с сердечно-сосудистыми и онкологическими заболеваниями. Основным объектом данного исследования как раз является один из таких типов бактерий – метициллинрезистентный золотистый стафилококк (MRSA), устойчивый к стандартным лекарственным препаратам, известным и применяемым на практике антибиотикам.

Инфицирование происходит просто и быстро, более того, такого рода заболевания относятся к внутрибольничным инфекциям или инфекциям, связанным с оказанием медицинской помощи (ИСМП), что также является насущной проблемой как для каждого из нас, так и для государства в целом.

Данной проблемой уже долгие годы занимаются ученые и медицинские организации по всему миру, и, наконец, с мертвой точки позволили сдвинуться силы искусственного интеллекта и глубокого обучения, основанного на применении графовых моделей нейронных сетей, знакомых для каждого из нас.

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Ученым из Массачусетского университета удалось осуществить данное исследование за счет использования глубокого обучения. Помимо того, что новый класс антибиотиков способен уничтожать MRSA бактерии, он также обладает очень низкой токсичностью по отношению к клеткам человека, что является безупречным результатом. Ключевым нововведением данного исследования является то, что ученые также смогли выяснить, какие виды информации использовала модель глубокого обучения для прогнозирования эффективности антибиотиков. Эти знания необходимы для дальнейшей разработки дополнительных препаратов, которые могли бы работать даже лучше, чем те, которые были определены моделью.

В данной статье я подробнее расскажу о методах и ходе данного исследования, от зарождения идеи, до ее реализации и практических результатов.

Приятного прочтения! :)

Путь от идеи до реализации исследования

Основной идеей исследователей являлось изучение химических пространств.

Химическое пространство – пространство потенциальных фармакологически активных молекул; его размер оценивается порядка 1060 молекул. Исследование химического пространства возможно путем создания “in silico” (или, проще говоря, “с помощью компьютерного моделирования (симуляции)”) баз данных виртуальных молекул, которые можно визуализировать, проецируя многомерное пространство свойств молекул в меньших измерениях.

Для исследования химических пространств используются подходы машинного обучения. Базовой моделью такого обучения является модель “черного ящика”, которая не обеспечивает понимания всех происходящих химических процессов в данном случае. Общий принцип заключается в том, что на выходе мы получаем готовый результат или принятое решение, однако используемые внутренние процессы и различные взвешенные факторы остаются неизвестными.

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Однако этого было недостаточно, т.к. понимание выборки конкретных химических соединений играло ключевую роль в прогнозировании дальнейших более эффективных структурных классов антибиотиков.

Исследователи разработали объяснимый, основанный на субструктурах подход для рационального исследования химических пространств с помощью глубокого обучения.

Субструктуры относятся к различным компонентам, модулям или слоям моделей глубокого обучения. Они представляют собой части модели, которые обрабатывают и извлекают информацию из входных данных. Субструктуры могут быть разными в зависимости от типа модели, но обычно они включают слои нейронной сети.

Кроме того, они обеспечивают "запоминание" важных шаблонов или связей в данных, что в конечном итоге способствует лучшим результатам прогнозирования или классификации. Это свойство субструктур и является основным в реализации исследования (для непосредственного понимания химических процессов).

Далее, благодаря использованию ансамблей графовых нейронных сетей, удалось предсказать антибиотическую активность и цитотоксичность для 12,076,365 соединений.

Ансамбль графовых нейронных сетей представляет собой комбинацию нескольких графовых нейронных сетей для решения задач анализа графов. Ансамбль графовых нейронных сетей объединяет прогнозы и предсказательные способности отдельных моделей графовых нейронных сетей для получения более надежных и точных результатов. Каждая модель в ансамбле может быть обучена на разных частях графов или с использованием различных гиперпараметров, что приводит к разнообразию в их предсказаниях.

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Таким образом, используя алгоритмы объясняемых графов, удалось на основании субструктур определить необходимые обоснования для выявленных соединений.

После получения обоснований итоговых результатов, эмпирически были протестированы 283 соединения. Обнаружили, что соединения, проявляющие антибиотическую активность, были обогащены предполагаемыми структурными классами, вытекающими из полученных обоснований.

В итоге, из этих структурных классов соединений один является селективным в отношении MRSA и ванкомицин-резистентных энтерококков, избегает значительной резистентности и снижает титры бактерий в мышиных моделях кожной и системной инфекции бедра, вызванной MRSA.

Теперь более детально рассмотрим особенности исследования!

Модели антибиотической активности

Был проверен исходный набор из 39 312 соединений, содержащий большинство известных антибиотиков, природных продуктов и структурно разнообразных молекул с молекулярными массами от 40 до 4200 Da, на ингибирующую рост активность против чувствительного к метициллину штамма S. aureus RN4220.

На графике отображается молекулярно-массовое распределение отобранных соединений. Частота показана в логарифмической шкале.
На графике отображается молекулярно-массовое распределение отобранных соединений. Частота показана в логарифмической шкале.

Исследование проводилось в течение ночи в богатой питательными веществами среде в конечной концентрации 50 мкМ, и их действие было бинаризировано как активное или неактивное при условии 80 % нормализованного подавления роста бактерии (начиная с 80% – активное), в результате чего было получено 512 активных соединений (1,3 % всех соединений).

Непосредственно данные по ингибированию роста S. aureus RN4220, полученные в результате анализа при конечной концентрации 50 мкМ. Данные получены в двух биологических репликах. Активные соединения – те, для которых средний относительный рост составляет менее 0,2.
Непосредственно данные по ингибированию роста S. aureus RN4220, полученные в результате анализа при конечной концентрации 50 мкМ. Данные получены в двух биологических репликах. Активные соединения – те, для которых средний относительный рост составляет менее 0,2.

Далее исследователи использовали Chemprop.

Chemprop - это метод машинного обучения, специально разработанный для задач химинформатики, в особенности для предсказания биологической активности новых соединений, поиска лекарственных препаратов, ранжирования молекул по их свойствам, генерации новых молекул и других задач, связанных с химической разработкой и оптимизацией.

Исследователи обучили ансамбли графовых нейронных сетей осуществлять бинарные классификационные прогнозы о том, будет ли новое соединение подавлять рост бактерий, на основе его химической структуры. Каждая графовая нейронная сеть работает путем формирования шагов свертки, которые зависят от атомов и связей каждой входной химической структуры, которая рассматривается как математический граф с вершинами (атомы) и ребрами (связи).

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После последовательных шагов свертки, объединяющих информацию от соседних атомов и связей, каждая модель генерирует окончательный балл предсказания между 0 и 1, представляющий собой оценку вероятности того, что молекула активна.

Для получения дополнительных данных, которые могут улучшить работу модели, каждой был предоставлен список молекулярных характеристик, рассчитанных с помощью RDKit для каждого входа.

RDKit – это набор программного обеспечения для химинформатики и машинного обучения, написанного на C++ и Python. RDKit позволяет выполнять различные операции над молекулами, включая генерацию, представление и редактирование молекулярных структур, расчет физико-химических свойств, числовых характеристик, описывающих молекулу в химической системе, визуализацию молекулярных структур, анализ и поиск структурных фрагментов, разработку моделей и тд.

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Для повышения надежности прогноза оценки нескольких моделей в ансамбле усреднялись. Каждая модель обучалась и проверялась, а затем тестировалась на одних и тех же фрагментах обучающего набора данных. Для ансамбля из десяти моделей, примененных к закрытым тестовым данным, площадь под кривой AUPRC составила 0,364, что свидетельствует о хорошей производительности при учете дисбаланса активных соединений в обучающих данных.

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Кривая AUPRC (Area Under the Precision-Recall Curve) - это графическое представление и метрика для оценки качества моделей классификации или ранжирования, особенно в задачах с несбалансированными классами или большим количеством ложноположительных результатов. Кривая AUPRC строится на основе двух основных метрик: precision (точность) и recall (полнота). Precision измеряет долю верно классифицированных положительных примеров относительно всех классифицированных положительных примеров. Recall измеряет долю верно классифицированных положительных примеров относительно всех действительно положительных примеров. Чем ближе AUPRC к значению 1, тем лучше модель способна разделять классы.

При этом, наблюдалось снижение производительности, измеряемой по AUPRC для тестового набора у альтернативных моделей, включая ансамбль из десяти моделей Chemprop без использования RDKit.

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В целом, эти результаты показывают, что модели Chemprop с молекулярными характеристиками, вычисленными с помощью RDKit, дают многообещающие прогнозы активности антибиотиков и могут превзойти более простые и поверхностные модели глубокого обучения.

Модели цитотоксичности человеческих клеток

Чтобы лучше определить соединения, селективные против изучаемых бактерий, были разработаны ортогональные модели, предсказывающие цитотоксичность в клетках человека.

Ортогональные модели являются специальным видом моделей, используемых в математике, статистике и анализе данных. Они характеризуются свойством ортогональности, что означает независимость и неперекрываемость компонентов модели. В контексте многомерного пространства, ортогональные модели связаны с ортогональными базисами, где каждая компонента (измерение) модели независима от остальных, и изменение одной компоненты не влияет на другие. Это обеспечивает более простой анализ данных и интерпретацию результатов.

Был проведен контрскрининг обучающего набора из 39,312 соединений на цитотоксичность в клетках карциномы печени человека (HepG2), клетках скелетных мышц человека (HSkMCs) и клетках фибробластов легких человека (IMR-90).

Клетки HepG2 обычно используются для изучения гепатотоксичности и общей цитотоксичности, в то время как клетки HSkMCs и IMR-90 могут лучше моделировать токсичность ”in vivo” (внутри клетки/живого организма), чем бессмертные клеточные линии (это клетки, которые способны продолжать делиться (продолжать жизненный цикл) бесконечно длительное время, в отличие от обычных клеток, которые имеют ограниченное количество делений и в конечном итоге умирают).

Жизнеспособность клеток измеряли после 2-3 дней обработки каждым соединением в концентрации 10 мкМ, которая соответствует и широко используется в культурах клеток человека. Затем активность соединений бинаризировали, используя строгий пороговый уровень жизнеспособности клеток в 90%, в результате чего в общей сложности 3341 (8,5%), 1490 (3,8%) и 3447 (8,8%) соединений были классифицированы как цитотоксичные для клеток HepG2, HSkMCs и IMR-90, соответственно, а из 512 активных антибактериальных соединений 306 оказались нецитотоксичными для всех трех типов клеток.

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Как и выше, эти данные были использованы для обучения бинарных классификационных моделей, предсказывающих вероятность того, является ли новое соединение цитотоксичным для клеток HepG2, HSkMCs или IMR-90, на основе химической структуры соединения. Для ансамблей из 10 моделей Chemprop, обученных и проверенных, а затем протестированных на тех же данных, значения AUPRC для моделей составили 0,176, 0,168 и 0,335, соответственно.

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Это свидетельствует о положительной, но менее предсказуемой эффективности, чем у моделей для антибиотической активности, что может быть обусловлено более строгими критериями для объявления соединений нецитотоксичными.

Фильтрация и визуализация химического пространства

После удовлетворения эффективностью наших моделей, исследователи переобучили ансамбли из 20 моделей Chemprop на всех обучающих наборах данных, в результате чего были получены четыре ансамбля, предсказывающие активность антибиотиков, цитотоксичность HepG2, цитотоксичность HSkMCs и цитотоксичность IMR-90. Ансамбли были применены для прогнозирования антибиотической активности и цитотоксичности 12 076 365 соединений, включая 11 277 225 соединений из базы данных Mcule purchasable, в которой большинство соединений можно легко приобрести, не прибегая к собственному химическому синтезу, а также 799 140 соединений из базы данных Broad Institute.

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Соединения с высокими (>0,4 для базы данных Mcule и >0,2 для базы данных Broad Institute) баллами прогноза антибиотической активности сохраняются, и аналогичные графовые нейронные сети применяются для прогнозирования цитотоксичности этих соединений для клеток HepG2, HSkMCs и клетки IMR-90. Соединения с низкими (<0,2) баллами прогноза цитотоксичности для всех типов клеток отбираются, затем вычислительным путем проверяются на наличие неблагоприятных химических подструктур (PAINS и Brenk substructures).

PAINS (Pan-Assay Interference Compounds) и Brenk substructures – это наборы подструктур, которые могут вызывать нежелательные эффекты или ложноположительные результаты в биологических экспериментах. PAINS является набором химических структурных фрагментов, которые часто обнаруживаются в молекулах, проявляющих побочную активность. Brenk substructures представляют собой схожий набор подструктур, разработанный для выявления химических групп, которые часто связаны с нежелательными свойствами молекул, такими как токсичность, незаурядность или проблемы с разработкой лекарств.

Наконец, оставшиеся соединения фильтруются на предмет структурной новизны, определяемой коэффициентом сходства Танимото – менее 0,5 по отношению к любому активному соединению в обучающем наборе данных.

Коэффициент Танимото является метрикой, используемой для оценки сходства между двумя множествами или наборами данных (преимущественно для молекул и генов). Коэффициент Танимото вычисляется как отношение числа общих элементов между двумя наборами к общему числу элементов в обоих наборах. Он может принимать значения в диапазоне от 0 до 1, где 0 означает полное отсутствие сходства, а 1 обозначает полное совпадение.

Формула для вычисления коэффициента Танимото:

T(A, B) = (A ∩ B) / (|A| + |B| - A ∩ B)

На основе полученных данных исследователи визуализировали химическое пространство, используя метод t-SNE.

t-SNE (t-distributed stochastic neighbor embedding) - это алгоритм машинного обучения, используемый для визуализации высокоразмерных данных в низкоразмерное пространство. Он позволяет отображать сложные многомерные данные на двух- или трехмерную плоскость, сохраняя при этом важные отношения и структуру в данных. Алгоритм t-SNE основывается на концепции близости объектов: близкие объекты в исходном пространстве имеют более высокие шансы быть близкими и после проекции на низкоразмерное пространство.

Диаграмма с использованием алгоритма t-SNE (t-distributed neighbor embedding) показывает соединения с высокими и низкими оценками прогноза антибиотиков, а также соединения из обучающего набора данных при разных пороговых значениях оценки прогноза. Данная диаграмма позволяет визуализировать химическую схожесть или различие представленных соединений. Активные соединения из обучающего набора данных (красные точки) в основном разделяют соединения с высокими оценками прогнозов (зеленые, черные и фиолетовые точки) от соединений с низкими оценками прогнозов (коричневые точки)
Диаграмма с использованием алгоритма t-SNE (t-distributed neighbor embedding) показывает соединения с высокими и низкими оценками прогноза антибиотиков, а также соединения из обучающего набора данных при разных пороговых значениях оценки прогноза. Данная диаграмма позволяет визуализировать химическую схожесть или различие представленных соединений. Активные соединения из обучающего набора данных (красные точки) в основном разделяют соединения с высокими оценками прогнозов (зеленые, черные и фиолетовые точки) от соединений с низкими оценками прогнозов (коричневые точки)

Особое замечание: как указано в t-SNE, несколько найденных веществ структурно отличаются от активных соединений в обучающем наборе, что указывает на то, что разработанные модели обобщаются на невидимые химические пространства.

Обоснование прогнозов классов антибиотиков

Так как графовые нейронные сети делают прогнозы на основе информации, содержащейся в атомах и связях каждой молекулы, исследователи предположили, что соединения с высокими оценками прогноза антибиотической активности содержат подструктуры («обоснования»), которые в значительной степени определяют их оценки.

Выявление таких обоснований обеспечило бы гарантии объяснимости модели для интересующих нас результатов. Оценка прогноза антибиотической активности для любого найденного вещества могла бы быть прямо отнесена к его обоснованию, так, что обоснование (или же рационал), рассматриваемое самостоятельно как молекулярный вход в Chemprop, сразу бы обладало высокой оценкой прогноза антибиотической активности. Возможность классификации таких обоснований сделала бы прогнозы Chemprop более понятными для людей и позволила бы последующий анализ подструктур с помощью машинного обучения.

Учитывая обученные модели Chemprop, удалось вычислить такие обоснования с помощью алгоритмов поиска на основе графов. Эти алгоритмы позволили определить в контексте одной молекулы наименьшее обоснование с заданным пороговым числом атомов, имеющее положительное предсказывающее значение.

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Исследователи стремились найти обоснования, содержащие не менее восьми атомов и проявляющие высокие предсказательные оценки антибиотической активности, превышающие 0,1, с использованием поисковых деревьев методом Монте-Карло.

Поиск методом Монте-Карло включает выбор начальной подструктуры, итеративное обрезание подструктуры и выбор удалений, приводящих к высоким оценкам предсказания, когда подграфы передаются в качестве входных данных в Chemprop.

Итеративное обрезание подструктуры – это метод оптимизации моделей глубокого обучения путем последовательного удаления нейронов или связей с низкой значимостью. В начале процесса все нейроны или связи обучаются на тренировочных данных. Затем производится оценка значимости каждого нейрона или связи - например, на основе их вклада в ошибку модели или значения градиентов. На основе этих оценок выбираются нейроны/связи с наименьшей значимостью и удаляются. После удаления происходит повторное обучение модели, чтобы компенсировать потерю ее емкости. Этот процесс повторяется, пока не достигнута желаемая эффективность модели.

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Примеры расчетов обоснования с использованием поиска по дереву Монте-Карло (Monte-Carlo Tree Search, MCTS):

a) Иллюстрация прямого прохода MCTS с использованием соединения 1. На рисунке показаны три возможных пути поиска от корня путем удаления периферийных связей или колец (выделены оранжевым). Из-за ограничений места показаны только три шага от корня.

b) Иллюстрация полного поискового пути от корня до листового узла (обоснование). Для предсказания активности каждого листового узла используется Chemprop, и эти предсказания используются для обновления статистики каждого промежуточного узла при обратном проходе.

Этот графический поиск выводит обоснование, объясняющее пороговое значение (не менее 0,1) предсказательной оценки соединения, если оно сходится; в противном случае, если обоснование не найдено, то интересующий нас случай нельзя объяснить таким образом.

Применяя этот анализ обоснований к отфильтрованным результатам полной модели, удалось вычислить обоснования для 380 из 3 646 выбранных соединений (10,4%). Как и ожидалось, многие рационалы совпали с известными фрагментами структурных классов. Однако также обнаружили обоснования, которые не были связаны ни с одним известным классом антибиотиков. Поэтому цель заключалась в лучшей фильтрации структурно новых интересных соединений и исследовании их соответствующих обоснований.

Новые фильтрованные подструктуры

Основываясь на выявлении известных классов антибиотиков в результате проведенных анализов, и способности графовых обоснований (то же, что и рационалы) предсказывать подструктуры, диагностирующие высокие показатели антибиотической активности, исследователи попытались выявить структурно новые классы антибиотиков, предсказанные обученными моделями.

Чтобы рассмотреть химические структуры с благоприятными свойствами для медицинской химии, исследователи удалили все найденные подструктуры, содержащие PAINS и Brenk-предупреждения, которые относятся к подструктурам, которые могут проявлять неспецифичную реакцию, мутагенность или иметь неблагоприятную фармакокинетику. Это уменьшило количество предсказанных вариантов с 3 646 до 2 209.

Далее исследователи сосредоточились на поиске соединений, не похожих на те, что были в обучающем наборе. Вычислили максимальное сходство по Танимото каждого соединения с любым активным соединением из обучающего набора и отобрали только с максимальным сходством ≤0,5 в качестве предварительной оценки.

В результате был получен окончательный набор из 1 261 совпадения, из которых 162 были из базы данных Mcule и 1 099 - из базы данных Broad Institute. При расчете обоснований для этого более узкого набора совпадений выяснилось, что 186 совпадений (14,8 %) имеют необходимые обоснования.

Теперь все исследования будут сосредоточены вокруг этих 186 совпадений.

Для того чтобы использовать эти рационалы для ясного прогнозирования структурных классов, предположили, что изучение химических основ, общих для рассматриваемых обоснований, позволит выделить наиболее значимые прогнозы структурных классов. Это особенно полезно для уменьшения объема выборки, поскольку типичные рационалы содержат большое количество атомов (более 17) и отличаются друг от друга незначительными модификациями. Были вычислены химические основы с хотя бы 12 атомами, которые сохранялись во всех обоснованиях.

С использованием этого подхода обнаружили, что 16 из 186 вариантов с нужными обоснованиями (8,6%) могут быть объединены с использованием 5 различных основ – G1-G5 – каждая группа содержит как минимум 2 варианта с соответствующими обоснованиями. Следует отметить, что три из пяти основ содержат хлор, что свидетельствует о том, что модели рассматривают присутствие атома хлора в этих химических контекстах как важный фактор, влияющий на антибиотическую активность.

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Здесь 16 соединений с необходимыми обоснованиями были связаны с пятью каркасами G1-G5.

Поскольку количество совпадений, оставшихся после фильтрации и анализа, было достаточно большим, исследователи напрямую проверили предсказания модели, получив девять совпадений, связанных с обоснованиями в группах G1-G5. Для сравнения также получили 45 совпадений (из отфильтрованных 1 261) с вычисленными обоснованиями, не связанными с G1-G5, 187 совпадений (из отфильтрованных 1 261) без вычисленных обоснований и 30 структурно несхожих соединений с низкими (менее 0,1) баллами предсказания. В результате такого подхода был получен набор из 283 соединений, которые экспериментально проверили.

Ранжированные оценки антибактериальной активности для 253 соединений с высокими (>0,2) оценками антибиотической активности и 30 соединений с низкими (<0,1) оценками антибиотической активности были запрошены для экспериментального тестирования.
Ранжированные оценки антибактериальной активности для 253 соединений с высокими (>0,2) оценками антибиотической активности и 30 соединений с низкими (<0,1) оценками антибиотической активности были запрошены для экспериментального тестирования.

Структурный класс антибиотиков из рационалов

При тестировании на ингибирование роста исследователи обнаружили, что 4 из 9 закупленных соединений (44%), связанных с группами G1-G5, проявляют активность против S. aureus с минимальными ингибирующими концентрациями (МИК) ≤ 32мкг/мл-1.

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Ни одно из 45 закупленных соединений с рационалами, не связанными с G1-G5, и 17 из 187 закупленных соединений без рационала (9,1%) не проявляли активности. Рабочие верные уровни обнаружения, связанные с всеми испытанными структурно новыми соединениями с рационалами (7,4%) и по всем испытанным структурно новым соединениям (8,7%), были выше доли активных соединений в обучающем наборе (1,3%), что указывает на полезность данного подхода. Эти значения указывают на то, что усилия по испытанию соединений могут быть так же продуктивными, как испытание случайных находок, когда они сосредоточены на структурных классах, предсказанных моделями глубокого обучения. Кроме того, в соответствии с низким уровнем ложного опускания для модели, ни одно из 30 закупленных соединений с низкими оценками предсказания не ингибировало рост S. aureus.

В итоге, из четырех найденных активных соединений, проявляющих активность против S. aureus и связанных с G1–G5, ни одно из них ранее не изучалось по отношению к рассматриваемым патогенам, и вместе они связаны с тремя группами рационалов — G1, G2 и G5. Стоит отметить, что G2 ассоциирована с двумя подтвержденными (активными) находками, что указывает на то, что эта группа рационалов может представлять активный структурный класс.

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*тут важно сконцентрировать внимание на соединениях 1 и 2, связанных с G2.

Дополнительные свойства, включая физико-химические наблюдения О'Ши и Мозера для антибиотиков, могут еще больше сузить химическое пространство и послужить основой для последующей разработки, особенно при рассмотрении кандидатов из больших библиотек. Однако исследователи не фильтровали находки на основе этих или других физико-химических свойств, при этом отмечается, что подтвержденные находки были меньше и менее полярны, чем типичные антибиотики для грамположительных бактерий.

Проведение дополнительных экспериментов по ингибированию роста показало, что соединения 1 и 2, а также почти все другие структурно новые подтвержденные находки также обладали активностью против MRSA USA300, сравнимым с его метициллиночувствительными аналогами. При контрсортировке их на цитотоксичность по отношению к клеткам HepG2, HSkMCs и IMR-90 мы обнаружили, что 20 из 21 структурно новых подтвержденных находок были нецитотоксичны при концентрации 10 мкМ. Соединения 1 и 2 проявляли значение полумаксимальной ингибирующей концентрации (IC50) ≥ 128 мкг/мл-1 для всех типов клеток, что указывает на прочную селективность против S. aureus.

В качестве окончательного эмпирического фильтра исследователи измерили МИК (минимальную ингибирующую концентрацию) S. aureus подтвержденных находок, связанных с G1-G5, в среде с добавлением 10% фетальной телячьей сыворотки в качестве контроля связывания соединений с белками сыворотки. Обнаружили, что МИК соединений 1 и 2 возросли в 4-8 раз до 16 мкг/мл-1, но оставались существенно ниже их значений IC50 для клеток человека. В отличие от этого, МИК других двух соединений увеличилась до ≥64 мкг/мл-1 в сыворотке. Вместе с их выгодными значениями МИК в среде без сыворотки (≥64-кратно меньше их значений IC50 для клеток человека), эти наблюдения указывали на то, что соединения 1 и 2 были наиболее селективными из всех подтвержденных находок и требовали дальнейшего изучения.

Механизм действия и резистентность

Для дальнейшего изучения отобранных соединений ученые дополнительно исследовали механизмы действия этих соединений с помощью эволюции устойчивых мутантов. Последовательно пассировали S. aureus RN4220, обработанный каждым из соединений 1 и 2 в жидкой культуре, и обнаружили, что их МИК оставались практически неизменными через 30 дней. Кроме того, исследователи высевали S. aureus RN4220 с высокой инокуляцией на твердую среду в присутствии сверхмикроскопических уровней соединений 1 и 2 и обнаружили, что через 5 дней колонии росли при 4×, но не 8×, что свидетельствует о низком уровне резистентности (устойчивости; частота резистентности при 4× МИК, ~10-8).

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Для дальнейшего изучения фенотипических эффектов (наблюдаемых свойств или характеристик) соединений 1 и 2 исследователи объединили микроскопические наблюдения с измерениями клеточной физиологии.

Сначала провели визуализацию одноклеточных; здесь исследователи сосредоточились на B. subtilis, чья палочковидная форма демонстрирует более заметные морфологические изменения, чем S. aureus. Одноклеточная визуализация показала, что клетки, обработанные соединением 1 или 2 лизировались (клетки разрушились, их мембраны разорвались или проникли вещества, приводящие к гибели клеток), что согласуется с бактерицидной активностью этих соединений и свидетельствует о механизме действия, направленном на клеточную оболочку.

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Кроме того, ученые обнаружили, что оба соединения были активны против 40 изолятов различных видов бактерий, выделенных Центром по контролю и профилактике заболеваний США (CDC), с различными факторами устойчивости, включая устойчивость к ванкомицину, аминогликозидам/тетрациклинам и оксазолидинону.

Следует отметить, что оба соединения были активны в отношении ванкомицинрезистентных энтерококков (VRE), представляющих серьезную угрозу устойчивости к антимикробным препаратам. Более того, эксперименты по уничтожению бактерий по времени показали, что оба соединения были эффективны против персистирующих (длительно существующих в организме хозяина) B. subtilis, что приводило к уничтожению культуры в лог-фазе после обработки канамицином. Эти результаты свидетельствуют о том, что соединения 1 и 2 способны преодолевать общие детерминанты устойчивости и толерантности к антибиотикам у грамположительных бактерий.

Свойства и эффективность in vivo

Исследователи обнаружили, что соединения 1 и 2 не являются гемолитическими, не хелатируют железо, не генотоксичны, химически стабильны в растворах с различным pH и нетоксичны при местном нанесении (1%) на кожу человека и внутрибрюшинном введении (80 мг кг−1 ) мышам.

Вывод

Основная цель исследования была достигнута – удалось выявить структурный класс антибиотиков, эффективный как при местном, так и при системном лечении MRSA в мышиных моделях инфекции.

Кроме того, эта работа представляет подход глубокого обучения, который систематически основывается на прогнозах отдельных составных соединений и позволяет эффективно исследовать огромные химические пространства на основе подструктур. В дополнение к уменьшению основного химического пространства, полезной особенностью данного подхода является возможность автоматизированного определения структурных мотивов. Эта возможность представляет собой источник химической новизны, который может предложить химические пространства для исследования и продуктивно дополнить существующие процессы открытия препаратов, например, путем генерации интересных химических фрагментов.

Исследователи предполагают, что лучшее понимание предсказаний на основе графовой обоснованности может помочь в открытии и проектировании дополнительных, крайне необходимых классов антибиотиков, например, таких, которые активны против грамотрицательных бактерий, а также классов препаратов, нацеленных на другие биологические процессы и заболевания, включая антивирусные и противораковые лекарства.

Таким образом, идея и необходимость ухода от “черного ящика” стала движущей силой для новых проектов в области биоинформатики.

Для меня исключительной важностью обладает внимание ученых, работающих с ИИ и моделями глубокого обучения, к насущным проблемам медицины и здравоохранения. Во многих вопросах мы оказываемся слишком бессильны, применяя стандартные методы исследования. Такие проекты помогают находить инновационные решения, без которых дальнейшая работа над решением проблем попросту невозможна или займет непростительно много времени. Можно с уверенностью сказать, что искусственный интеллект на страже нового здорового поколения! :)

На этом всё!

Будем ждать Вас в комментариях! Спасибо за прочтение :)

Автор: Черенковская Анастасия

Источник

  • 01.09.25 17:03 WILLER GOODY

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  • 05.09.25 15:39 micheal1219

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  • 08.09.25 14:26 bajo171

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  • 09.09.25 20:37 DAVEortega1010

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  • 10.09.25 00:45 robertalfred175

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  • 10.09.25 00:45 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 11.09.25 03:31 DAVEortega1010

    Be wary of fraudulent cryptocurrency recovery services. Many promise to recover your lost digital currency. These services often provide excuses or fail to deliver any results. This only adds to your distress. After several unsuccessful attempts with others, I discovered a genuine expert. This individual was highly capable and provided excellent assistance. They successfully recovered my stolen cryptocurrency. I was finally able to find peace of mind. If you have lost crypto, you should contact them. talk to [email protected] for help. You can also send a text message via WhatsApp to +447366445035, Stop losing time on scams. They can help you get your money back.

  • 11.09.25 13:44 grace

    i fell for an investment scam also and lost $250k worth of bitcoin luckily i found anthonydavies01 on telegram with his assistance i got back over 85% of my money. him and his team are top notch

  • 11.09.25 15:03 stevensjonas

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  • 11.09.25 18:51 patricialovick86

    How To Recover Your Bitcoin Without Falling Victim To Scams: A  Testimony Experience With Capital Crypto Recover Services, Contact Telegram: @Capitalcryptorecover Dear Everyone, I would like to take a moment to share my positive experience with Capital Crypto Recover Services. Initially, I was unsure if it would be possible to recover my stolen bitcoins. However, with their expertise and professionalism, I was able to fully recover my funds. Unfortunately, many individuals fall victim to scams in the cryptocurrency space, especially those involving fraudulent investment platforms. However, I advise caution, as not all recovery services are legitimate. I personally lost $273,000 worth of Bitcoin from my Binance account due to a deceptive platform. If you have suffered a similar loss, you may be considering crypto recovery, The Capital Crypto Recover is the most knowledgeable and effective Capital Crypto Recovery Services assisted me in recovering my stolen funds within 24 hours, after getting access to my wallet. Their service was not only prompt but also highly professional and effective, and many recovery services may not be trustworthy. Therefore, I highly recommend Capital Crypto Recover to you. i do always research and see reviews about their service, For assistance finding your misplaced cryptocurrency, get in touch with them, They do their jobs quickly and excellently, Stay safe and vigilant in the crypto world. Contact: [email protected]  You can reach them via email at [email protected] OR Call/Text Number +1 (336)390-6684 his contact website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 11.09.25 18:51 patricialovick86

    How To Recover Your Bitcoin Without Falling Victim To Scams: A  Testimony Experience With Capital Crypto Recover Services, Contact Telegram: @Capitalcryptorecover Dear Everyone, I would like to take a moment to share my positive experience with Capital Crypto Recover Services. Initially, I was unsure if it would be possible to recover my stolen bitcoins. However, with their expertise and professionalism, I was able to fully recover my funds. Unfortunately, many individuals fall victim to scams in the cryptocurrency space, especially those involving fraudulent investment platforms. However, I advise caution, as not all recovery services are legitimate. I personally lost $273,000 worth of Bitcoin from my Binance account due to a deceptive platform. If you have suffered a similar loss, you may be considering crypto recovery, The Capital Crypto Recover is the most knowledgeable and effective Capital Crypto Recovery Services assisted me in recovering my stolen funds within 24 hours, after getting access to my wallet. Their service was not only prompt but also highly professional and effective, and many recovery services may not be trustworthy. Therefore, I highly recommend Capital Crypto Recover to you. i do always research and see reviews about their service, For assistance finding your misplaced cryptocurrency, get in touch with them, They do their jobs quickly and excellently, Stay safe and vigilant in the crypto world. Contact: [email protected]  You can reach them via email at [email protected] OR Call/Text Number +1 (336)390-6684 his contact website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 11.09.25 18:51 patricialovick86

    How To Recover Your Bitcoin Without Falling Victim To Scams: A  Testimony Experience With Capital Crypto Recover Services, Contact Telegram: @Capitalcryptorecover Dear Everyone, I would like to take a moment to share my positive experience with Capital Crypto Recover Services. Initially, I was unsure if it would be possible to recover my stolen bitcoins. However, with their expertise and professionalism, I was able to fully recover my funds. Unfortunately, many individuals fall victim to scams in the cryptocurrency space, especially those involving fraudulent investment platforms. However, I advise caution, as not all recovery services are legitimate. I personally lost $273,000 worth of Bitcoin from my Binance account due to a deceptive platform. If you have suffered a similar loss, you may be considering crypto recovery, The Capital Crypto Recover is the most knowledgeable and effective Capital Crypto Recovery Services assisted me in recovering my stolen funds within 24 hours, after getting access to my wallet. Their service was not only prompt but also highly professional and effective, and many recovery services may not be trustworthy. Therefore, I highly recommend Capital Crypto Recover to you. i do always research and see reviews about their service, For assistance finding your misplaced cryptocurrency, get in touch with them, They do their jobs quickly and excellently, Stay safe and vigilant in the crypto world. Contact: [email protected]  You can reach them via email at [email protected] OR Call/Text Number +1 (336)390-6684 his contact website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 12.09.25 13:11 grace

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  • 12.09.25 15:06 michaeldavenport218

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  • 12.09.25 15:06 michaeldavenport218

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  • 13.09.25 20:45 Clinton

    I know firsthand how devastating it feels to lose money to a scam. When it happened to me, I was left frustrated, hopeless, and unsure where to turn. Complaining to apps about being scammed on their platforms didn’t change anything it felt like no one cared. But through a friend’s advice, I connected with a recovery professional who gave me real hope. With their help, I was able to get my funds back, something I thought was impossible. I’m sharing this because I don’t want anyone else to feel as lost as I once did. Whether you’ve been scammed through crypto platforms, dating scams, real-estate or mortgage fraud, or even fake ICOs, please know there’s still a way forward. If you’ve lost money, reach out to Recoveryfundprovider(@)gmail(.)com).) or (WhatsApp) at (+)44 7366445035 for consultation and recovery services.

  • 14.09.25 00:54 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 14.09.25 00:54 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 14.09.25 02:37 luciajessy3

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  • 14.09.25 02:37 luciajessy3

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  • 14.09.25 12:44 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 14.09.25 12:44 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 14.09.25 15:13 estherfords

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  • 14.09.25 15:13 estherfords

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  • 14.09.25 17:48 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 14.09.25 17:48 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 14.09.25 20:49 Clinton

    I know firsthand how devastating it feels to lose money to a scam. When it happened to me, I was left frustrated, hopeless, and unsure where to turn. Complaining to apps about being scammed on their platforms didn’t change anything it felt like no one cared. But through a friend’s advice, I connected with a recovery professional who gave me real hope. With their help, I was able to get my funds back, something I thought was impossible. I’m sharing this because I don’t want anyone else to feel as lost as I once did. Whether you’ve been scammed through crypto platforms, dating scams, real-estate or mortgage fraud, or even fake ICOs, please know there’s still a way forward. If you’ve lost money, reach out to ([email protected]) or (WhatsApp) at (+)44 7366445035 for consultation and recovery services.

  • 14.09.25 22:16 Berrysmith

    I recently recovered a large sum of digital assets. Sylvester Bryant, reachable at Yt7cracker@gmail. com, helped me reclaim 720,000 in Ethereum. The entire experience was excellent. I highly recommend Mr. Sylvester. He assists those dealing with lost or stolen funds. His skill in getting back money from scams is outstanding. Scammers cause huge stress and financial pain. Mr. Bryant offers a crucial service. He provides a way to possibly recover stolen funds. His professional manner built trust. If you lost money to scams, contact him. He is also on WhatsApp at +1 512 577 7957. This makes it easy to discuss your case. Recovering this amount was difficult. Mr. Bryant showed deep knowledge of the technical work. His commitment to solving these problems is clear. Seek his help for lost crypto or other digital assets. This is a major concern for many. Expert aid can greatly improve outcomes.

  • 15.09.25 10:25 aliceforemanlaw

    I got back a lot of digital money recently. Sylvester Bryant helped me recover 120,000 in Ethereum. You can reach him at Yt7cracker@gmail. com. It was a great experience overall. I really suggest Mr. Sylvester. He helps people who lost money. He is great at getting money back from scams. Scammers cause a lot of stress and hurt. Mr. Bryant offers an important service. He can help you get back stolen funds. He was very professional and earned my trust. Contact him if you lost money to scams. He is also on WhatsApp at +1 512 577 7957. This makes it easy to talk about your situation. Getting this amount back was tough. Mr. Bryant knew a lot about the technical parts. His drive to fix these issues is clear. Get his help if you lost crypto or other digital items. Many people worry about this. Getting expert help can really make a difference.

  • 15.09.25 12:49 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 15.09.25 12:49 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 15.09.25 12:49 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 16.09.25 12:25 aliceforemanlaw

    Sylvester Bryant helped me recover 120,000 in Ethereum. You can reach him at Yt7cracker@gmail. com. It was a great experience overall. I really suggest Mr. Sylvester. He helps people who lost money. He is great at getting money back from scams. Scammers cause a lot of stress and hurt. Mr. Bryant offers an important service. He can help you get back stolen funds. He was very professional and earned my trust. Contact him if you lost money to scams. He is also on WhatsApp at +1 512 577 7957. This makes it easy to talk about your situation. Getting this amount back was tough. Mr. Bryant knew a lot about the technical parts. His drive to fix these issues is clear. Get his help if you lost crypto or other digital items. Many people worry about this. Getting expert help can really make a difference.

  • 16.09.25 12:26 aliceforemanlaw

    Sylvester Bryant helped me recover 120,000 in Ethereum. You can reach him at Yt7cracker@gmail. com. It was a great experience overall. I really suggest Mr. Sylvester. He helps people who lost money. He is great at getting money back from scams. Scammers cause a lot of stress and hurt. Mr. Bryant offers an important service. He can help you get back stolen funds. He was very professional and earned my trust. Contact him if you lost money to scams. He is also on WhatsApp at +1 512 577 7957. This makes it easy to talk about your situation. Getting this amount back was tough. Mr. Bryant knew a lot about the technical parts. His drive to fix these issues is clear. Get his help if you lost crypto or other digital items. Many people worry about this. Getting expert help can really make a difference.

  • 16.09.25 13:25 marcushenderson624

    Bitcoin Recovery Testimonial After falling victim to a cryptocurrency scam group, I lost $354,000 worth of USDT. I thought all hope was lost from the experience of losing my hard-earned money to scammers. I was devastated and believed there was no way to recover my funds. Fortunately, I started searching for help to recover my stolen funds and I came across a lot of testimonials online about Capital Crypto Recovery, an agent who helps in recovery of lost bitcoin funds, I contacted Capital Crypto Recover Service, and with their expertise, they successfully traced and recovered my stolen assets. Their team was professional, kept me updated throughout the process, and demonstrated a deep understanding of blockchain transactions and recovery protocols. They are trusted and very reliable with a 100% successful rate record Recovery bitcoin, I’m grateful for their help and highly recommend their services to anyone seeking assistance with lost crypto. Contact: [email protected] Phone CALL/Text Number: +1 (336) 390-6684 Email: [email protected] Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 16.09.25 13:25 marcushenderson624

    Bitcoin Recovery Testimonial After falling victim to a cryptocurrency scam group, I lost $354,000 worth of USDT. I thought all hope was lost from the experience of losing my hard-earned money to scammers. I was devastated and believed there was no way to recover my funds. Fortunately, I started searching for help to recover my stolen funds and I came across a lot of testimonials online about Capital Crypto Recovery, an agent who helps in recovery of lost bitcoin funds, I contacted Capital Crypto Recover Service, and with their expertise, they successfully traced and recovered my stolen assets. Their team was professional, kept me updated throughout the process, and demonstrated a deep understanding of blockchain transactions and recovery protocols. They are trusted and very reliable with a 100% successful rate record Recovery bitcoin, I’m grateful for their help and highly recommend their services to anyone seeking assistance with lost crypto. Contact: [email protected] Phone CALL/Text Number: +1 (336) 390-6684 Email: [email protected] Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 17.09.25 02:56 peju1213

    It can be very concerning to lose access to your USDT (Tether) wallet. It could potentially result in permanent loss of significant funds. You may have misplaced your private keys. Perhaps you unintentionally erased something, or your security was breached. It seems hopeless when you are unable to access your USDT. However, it doesn't have to stop there. There are methods for recovering your lost USDT. During difficult circumstances, professional assistance and wise recuperation techniques can give you hope. get in touch with Marie ([email protected] and +1 7127594675) on WhatsApp.

  • 17.09.25 11:16 [email protected]

    Sylvester Bryant helped me recover $510,000 in Ethereum, and I highly recommend his services. He specializes in helping people recover funds lost to scams. Scammers can cause significant stress, but Mr. Bryant's professionalism and expertise in the technical aspects of recovery earned my trust. If you've lost money through scams, you can reach him at [ yt7cracker@gmail. com ] or on WhatsApp at +1 512 577 7957. His dedication to helping people get their stolen funds back is clear, and his support made a huge difference in my case.

  • 17.09.25 11:16 [email protected]

    Sylvester Bryant helped me recover $510,000 in Ethereum, and I highly recommend his services. He specializes in helping people recover funds lost to scams. Scammers can cause significant stress, but Mr. Bryant's professionalism and expertise in the technical aspects of recovery earned my trust. If you've lost money through scams, you can reach him at [ yt7cracker@gmail. com ] or on WhatsApp at +1 512 577 7957. His dedication to helping people get their stolen funds back is clear, and his support made a huge difference in my case.

  • 17.09.25 23:50 marcushenderson624

    Bitcoin Recovery Testimonial After falling victim to a cryptocurrency scam group, I lost $354,000 worth of USDT. I thought all hope was lost from the experience of losing my hard-earned money to scammers. I was devastated and believed there was no way to recover my funds. Fortunately, I started searching for help to recover my stolen funds and I came across a lot of testimonials online about Capital Crypto Recovery, an agent who helps in recovery of lost bitcoin funds, I contacted Capital Crypto Recover Service, and with their expertise, they successfully traced and recovered my stolen assets. Their team was professional, kept me updated throughout the process, and demonstrated a deep understanding of blockchain transactions and recovery protocols. They are trusted and very reliable with a 100% successful rate record Recovery bitcoin, I’m grateful for their help and highly recommend their services to anyone seeking assistance with lost crypto. Contact: [email protected] Phone CALL/Text Number: +1 (336) 390-6684 Email: [email protected] Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 17.09.25 23:50 marcushenderson624

    Bitcoin Recovery Testimonial After falling victim to a cryptocurrency scam group, I lost $354,000 worth of USDT. I thought all hope was lost from the experience of losing my hard-earned money to scammers. I was devastated and believed there was no way to recover my funds. Fortunately, I started searching for help to recover my stolen funds and I came across a lot of testimonials online about Capital Crypto Recovery, an agent who helps in recovery of lost bitcoin funds, I contacted Capital Crypto Recover Service, and with their expertise, they successfully traced and recovered my stolen assets. Their team was professional, kept me updated throughout the process, and demonstrated a deep understanding of blockchain transactions and recovery protocols. They are trusted and very reliable with a 100% successful rate record Recovery bitcoin, I’m grateful for their help and highly recommend their services to anyone seeking assistance with lost crypto. Contact: [email protected] Phone CALL/Text Number: +1 (336) 390-6684 Email: [email protected] Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 17.09.25 23:50 marcushenderson624

    Bitcoin Recovery Testimonial After falling victim to a cryptocurrency scam group, I lost $354,000 worth of USDT. I thought all hope was lost from the experience of losing my hard-earned money to scammers. I was devastated and believed there was no way to recover my funds. Fortunately, I started searching for help to recover my stolen funds and I came across a lot of testimonials online about Capital Crypto Recovery, an agent who helps in recovery of lost bitcoin funds, I contacted Capital Crypto Recover Service, and with their expertise, they successfully traced and recovered my stolen assets. Their team was professional, kept me updated throughout the process, and demonstrated a deep understanding of blockchain transactions and recovery protocols. They are trusted and very reliable with a 100% successful rate record Recovery bitcoin, I’m grateful for their help and highly recommend their services to anyone seeking assistance with lost crypto. Contact: [email protected] Phone CALL/Text Number: +1 (336) 390-6684 Email: [email protected] Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 18.09.25 11:55 Mundo

    Sylvester Bryant recovered £400,000 in Ethereum for me. I strongly advise using his services. He focuses on retrieving money lost to fraudulent schemes. Scams cause immense worry. Mr. Bryant's expert knowledge and calm approach in getting funds back reassured me. If you have lost money to scams, contact him via email at [email protected]. You can also reach him on WhatsApp at +1 512 577 7957. He is committed to helping people recover their stolen assets. His support was invaluable in my situation.

  • 18.09.25 11:55 Mundo

    Sylvester Bryant recovered £400,000 in Ethereum for me. I strongly advise using his services. He focuses on retrieving money lost to fraudulent schemes. Scams cause immense worry. Mr. Bryant's expert knowledge and calm approach in getting funds back reassured me. If you have lost money to scams, contact him via email at [email protected]. You can also reach him on WhatsApp at +1 512 577 7957. He is committed to helping people recover their stolen assets. His support was invaluable in my situation.

  • 18.09.25 12:22 wendytaylor015

    My name is Wendy Taylor, I'm from Los Angeles, i want to announce to you Viewer how Capital Crypto Recover help me to restore my Lost Bitcoin, I invested with a Crypto broker without proper research to know what I was hoarding my hard-earned money into scammers, i lost access to my crypto wallet or had your funds stolen? Don’t worry Capital Crypto Recover is here to help you recover your cryptocurrency with cutting-edge technical expertise, With years of experience in the crypto world, Capital Crypto Recover employs the best latest tools and ethical hacking techniques to help you recover lost assets, unlock hacked accounts, Whether it’s a forgotten password, Capital Crypto Recover has the expertise to help you get your crypto back. a security company service that has a 100% success rate in the recovery of crypto assets, i lost wallet and hacked accounts. I provided them the information they requested and they began their investigation. To my surprise, Capital Crypto Recover was able to trace and recover my crypto assets successfully within 24hours. Thank you for your service in helping me recover my $647,734 worth of crypto funds and I highly recommend their recovery services, they are reliable and a trusted company to any individuals looking to recover lost money. Contact email [email protected] OR Telegram @Capitalcryptorecover Call/Text Number +1 (336)390-6684 his contact: [email protected] His website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 18.09.25 12:22 wendytaylor015

    My name is Wendy Taylor, I'm from Los Angeles, i want to announce to you Viewer how Capital Crypto Recover help me to restore my Lost Bitcoin, I invested with a Crypto broker without proper research to know what I was hoarding my hard-earned money into scammers, i lost access to my crypto wallet or had your funds stolen? Don’t worry Capital Crypto Recover is here to help you recover your cryptocurrency with cutting-edge technical expertise, With years of experience in the crypto world, Capital Crypto Recover employs the best latest tools and ethical hacking techniques to help you recover lost assets, unlock hacked accounts, Whether it’s a forgotten password, Capital Crypto Recover has the expertise to help you get your crypto back. a security company service that has a 100% success rate in the recovery of crypto assets, i lost wallet and hacked accounts. I provided them the information they requested and they began their investigation. To my surprise, Capital Crypto Recover was able to trace and recover my crypto assets successfully within 24hours. Thank you for your service in helping me recover my $647,734 worth of crypto funds and I highly recommend their recovery services, they are reliable and a trusted company to any individuals looking to recover lost money. Contact email [email protected] OR Telegram @Capitalcryptorecover Call/Text Number +1 (336)390-6684 his contact: [email protected] His website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 18.09.25 13:04 Mundo

    Sylvester Bryant recovered £400,000 in Ethereum for me. I strongly advise using his services. He focuses on retrieving money lost to fraudulent schemes. Scams cause immense worry. Mr. Bryant's expert knowledge and calm approach in getting funds back reassured me. If you have lost money to scams, contact him via email at [email protected]. You can also reach him on WhatsApp at +1 512 577 7957. He is committed to helping people recover their stolen assets. His support was invaluable in my situation.

  • 18.09.25 16:46 carolinehudso83

    I noticed a lot of online recommendations, and it’s clear that some of them are bad eggs that will just make уour mуsterу worse. I can onlу suggest one, and if уou need assistance getting back the moneу уou lost to scammers, уou can contact them bу email at: [email protected] Whats app or text +1 518 468 2985 Website https://bestrecoveryagent.com/

  • 19.09.25 00:54 frank2025

    Anthony davies played a crucial role in successfully recovering my stolen USDT, which was valued at more than $300,000. His expertise and dedication made the recovery process smooth and efficient. Throughout the entire time, he demonstrated himself to be a reliable recovery agent. His professionalism and commitment to his clients are evident in the results he achieves. If you find yourself in a similar situation or need assistance, he is available for contact. You can reach anthony through email at (anthonydaviestech AT gmail dot com). Alternatively, he is also accessible via telegram at anthonydavies01. Reaching out to him could be the first step toward recovering your lost assets

  • 19.09.25 10:02 faithlawrence

    Sylvester Bryant recovered £400,000 in Ethereum for me. I strongly advise using his services. He focuses on retrieving money lost to fraudulent schemes. Scams cause immense worry. Mr. Bryant's expert knowledge and calm approach in getting funds back reassured me. If you have lost money to scams, contact him via email at [email protected]. You can also reach him on WhatsApp at +1 512 577 7957. He is committed to helping people recover their stolen assets. His support was invaluable in my situation.

  • 19.09.25 10:02 faithlawrence

    Sylvester Bryant recovered £400,000 in Ethereum for me. I strongly advise using his services. He focuses on retrieving money lost to fraudulent schemes. Scams cause immense worry. Mr. Bryant's expert knowledge and calm approach in getting funds back reassured me. If you have lost money to scams, contact him via email at [email protected]. You can also reach him on WhatsApp at +1 512 577 7957. He is committed to helping people recover their stolen assets. His support was invaluable in my situation.

  • 19.09.25 15:27 tonykith01477

    There are not many reviews about free crypto recovery fixed but the ones that exist are mostly positive. I have no idea how that is possible, but it is clear that they are fabricated. However, there are also negative reviews – and I highly recommend paying attention to them. These are real people describing their unfortunate experiences with this scam. It’s the same story as always – fraudsters create a fake website to con people out of money. Nothing new but look no more and contact FASTRECOVERYAGENT These types of scams are everywhere. If you have already lost money to scammers i mean any type be it sending to scammers account , or if you have a case about stolen bitcoin , usdt, or any type of cryptocurrency whatsoever just reach out to www.fastrecoveryagent.com, i really owe this group of recovery expert because they saved me after bad investment with a fake broker, i literally owe them my life as they saved me from some bunch of scammers, but see how life is , i got every cent of my investment back with the profit , reach out to to them today and tell them from Mary because i promise them i will tell the whole world when the recovery is complete, and here i go after i got my recovered assets thats why im doing what i promised. do not fall for another scam. contact the,m today!!!!

  • 19.09.25 17:12 wendytaylor015

    My name is Wendy Taylor, I'm from Los Angeles, i want to announce to you Viewer how Capital Crypto Recover help me to restore my Lost Bitcoin, I invested with a Crypto broker without proper research to know what I was hoarding my hard-earned money into scammers, i lost access to my crypto wallet or had your funds stolen? Don’t worry Capital Crypto Recover is here to help you recover your cryptocurrency with cutting-edge technical expertise, With years of experience in the crypto world, Capital Crypto Recover employs the best latest tools and ethical hacking techniques to help you recover lost assets, unlock hacked accounts, Whether it’s a forgotten password, Capital Crypto Recover has the expertise to help you get your crypto back. a security company service that has a 100% success rate in the recovery of crypto assets, i lost wallet and hacked accounts. I provided them the information they requested and they began their investigation. To my surprise, Capital Crypto Recover was able to trace and recover my crypto assets successfully within 24hours. Thank you for your service in helping me recover my $647,734 worth of crypto funds and I highly recommend their recovery services, they are reliable and a trusted company to any individuals looking to recover lost money. Contact email [email protected] OR Telegram @Capitalcryptorecover Call/Text Number +1 (336)390-6684 his contact: [email protected] His website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 19.09.25 17:12 wendytaylor015

    My name is Wendy Taylor, I'm from Los Angeles, i want to announce to you Viewer how Capital Crypto Recover help me to restore my Lost Bitcoin, I invested with a Crypto broker without proper research to know what I was hoarding my hard-earned money into scammers, i lost access to my crypto wallet or had your funds stolen? Don’t worry Capital Crypto Recover is here to help you recover your cryptocurrency with cutting-edge technical expertise, With years of experience in the crypto world, Capital Crypto Recover employs the best latest tools and ethical hacking techniques to help you recover lost assets, unlock hacked accounts, Whether it’s a forgotten password, Capital Crypto Recover has the expertise to help you get your crypto back. a security company service that has a 100% success rate in the recovery of crypto assets, i lost wallet and hacked accounts. I provided them the information they requested and they began their investigation. To my surprise, Capital Crypto Recover was able to trace and recover my crypto assets successfully within 24hours. Thank you for your service in helping me recover my $647,734 worth of crypto funds and I highly recommend their recovery services, they are reliable and a trusted company to any individuals looking to recover lost money. Contact email [email protected] OR Telegram @Capitalcryptorecover Call/Text Number +1 (336)390-6684 his contact: [email protected] His website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 20.09.25 15:05 faithlawrence

    I can’t recommend Sylvester Bryant enough. He successfully recovered €210,000 in Ethereum for me after I had lost hope. His expertise in tracing and reclaiming funds from scams is truly remarkable. What stood out most was his calm, professional approach and deep technical knowledge, which gave me complete confidence throughout the process. If you’ve lost money to a scam, I strongly encourage you to reach out to him. You can contact him via email at [[email protected] ] or on WhatsApp at +1 512 577 7957. He is genuinely dedicated to helping people recover what they’ve lost, and his support has been invaluable to me.

  • 20.09.25 15:53 blessing1198

    It is distressing to lose USDT due to a bitcoin wallet hack. Although it is challenging, stolen USDT can be recovered. Taking prompt, wise action increases your chances. Marie can help you with reporting the theft, what to do right away, and USDT recovery. Contact her on WhatsApp at +1 7127594675, or email [email protected].

  • 21.09.25 12:05 star1121

    Consider this: In cryptocurrency, you see what appears to be a fantastic opportunity. After investing your hard-earned money, you see it disappear into the wallet of a con artist. Your bank account seems empty as rage and regret merge in this stomach punch. These days, cryptocurrency frauds are very common, however recovering your cryptocurrency from scammers is still possible. You can track down and possibly recover what you lost with shrewd actions. Marie can help; contact her at [email protected] and on WhatsApp at +1 7127594675.

  • 23.09.25 03:23 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 23.09.25 03:23 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 23.09.25 03:23 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 23.09.25 03:54 robertalfred175

    CRYPTO SCAM RECOVERY SUCCESSFUL – A TESTIMONIAL OF LOST PASSWORD TO YOUR DIGITAL WALLET BACK. My name is Robert Alfred, Am from Australia. I’m sharing my experience in the hope that it helps others who have been victims of crypto scams. A few months ago, I fell victim to a fraudulent crypto investment scheme linked to a broker company. I had invested heavily during a time when Bitcoin prices were rising, thinking it was a good opportunity. Unfortunately, I was scammed out of $120,000 AUD and the broker denied me access to my digital wallet and assets. It was a devastating experience that caused many sleepless nights. Crypto scams are increasingly common and often involve fake trading platforms, phishing attacks, and misleading investment opportunities. In my desperation, a friend from the crypto community recommended Capital Crypto Recovery Service, known for helping victims recover lost or stolen funds. After doing some research and reading multiple positive reviews, I reached out to Capital Crypto Recovery. I provided all the necessary information—wallet addresses, transaction history, and communication logs. Their expert team responded immediately and began investigating. Using advanced blockchain tracking techniques, they were able to trace the stolen Dogecoin, identify the scammer’s wallet, and coordinate with relevant authorities to freeze the funds before they could be moved. Incredibly, within 24 hours, Capital Crypto Recovery successfully recovered the majority of my stolen crypto assets. I was beyond relieved and truly grateful. Their professionalism, transparency, and constant communication throughout the process gave me hope during a very difficult time. If you’ve been a victim of a crypto scam, I highly recommend them with full confidence contacting: 📧 Email: [email protected] 📱 Telegram: @Capitalcryptorecover Contact: [email protected] 📞 Call/Text: +1 (336) 390-6684 🌐 Website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 23.09.25 14:45 Raymondkrisitina

    Sylvester Bryant gets my full recommendation. He helped me get back €230,000 in Ethereum when I thought it was gone forever. His skills in tracking and retrieving scam-lost funds amaze me. I appreciated his steady, expert manner and strong tech know-how. That built my trust every step of the way. If a scam has taken your money, get in touch with him right away. Email him at yt7cracker@gmail. com or message on WhatsApp at +1 512 577 7957. He truly commits to aiding folks in regaining their assets. His help meant everything to me.

  • 23.09.25 19:27 beverlyalex010

    In 2022, I lost 1.5 ETH in what I thought was a promising Twitter investment. I reached out to the so-called brokers behind it, but they vanished and stopped replying. For years, I carried the heavy belief that my crypto was gone forever. Then, by chance, I discovered RecoveryFundProvider [email protected] WhatsApp at +44 736-644-5035 through a finance podcast. That moment changed everything. Their team didn’t lure me in with false promises or flashy guarantees. Instead, they explained their process clearly specializing in advanced blockchain tracing, smart contract forensics, and even transaction reversal in certain cases. Skeptical but hopeful, I allowed them to review my case. What followed was beyond my expectations. They dug deep into the blockchain ledger, followed the coin’s complex movements across multiple addresses, and even collaborated with global nodes to trace every trail. Two weeks later, I received an alert I never thought I’d see again: my crypto had been fully recovered and safely transferred back to me. I cried tears of relief. What sets RecoveryFundProvider apart isn’t just their cutting-edge expertise, but their unwavering ethics and dedication. They treat every client like more than just another case number even years after a loss. Today, I can confidently say they live up to their name, and I’m endlessly grateful.

  • 23.09.25 22:38 Raymondkrisitina

    Sylvester Bryant gets my full recommendation. He helped me get back €230,000 in Ethereum when I thought it was gone forever. His skills in tracking and retrieving scam-lost funds amaze me. I appreciated his steady, expert manner and strong tech know-how. That built my trust every step of the way. If a scam has taken your money, get in touch with him right away. Email him at yt7cracker@gmail. com or message on WhatsApp at +1 512 577 7957. He truly commits to aiding folks in regaining their assets. His help meant everything to me.

  • 24.09.25 12:22 beverlyalex010

    In 2022, I lost 1.5 ETH in what I thought was a promising Twitter investment. I reached out to the so-called brokers behind it, but they vanished and stopped replying. For years, I carried the heavy belief that my crypto was gone forever. Then, by chance, I discovered RecoveryFundProvider [email protected] WhatsApp at +44 736-644-5035 through a finance podcast. That moment changed everything. Their team didn’t lure me in with false promises or flashy guarantees. Instead, they explained their process clearly specializing in advanced blockchain tracing, smart contract forensics, and even transaction reversal in certain cases. Skeptical but hopeful, I allowed them to review my case. What followed was beyond my expectations. They dug deep into the blockchain ledger, followed the coin’s complex movements across multiple addresses, and even collaborated with global nodes to trace every trail. Two weeks later, I received an alert I never thought I’d see again: my crypto had been fully recovered and safely transferred back to me. I cried tears of relief. What sets RecoveryFundProvider apart isn’t just their cutting-edge expertise, but their unwavering ethics and dedication. They treat every client like more than just another case number even years after a loss. Today, I can confidently say they live up to their name, and I’m endlessly grateful.

  • 24.09.25 15:50 michaeldavenport218

    I was recently scammed out of $53,000 by a fraudulent Bitcoin investment scheme, which added significant stress to my already difficult health issues, as I was also facing cancer surgery expenses. Desperate to recover my funds, I spent hours researching and consulting other victims, which led me to discover the excellent reputation of Capital Crypto Recover, I came across a Google post It was only after spending many hours researching and asking other victims for advice that I discovered Capital Crypto Recovery’s stellar reputation. I decided to contact them because of their successful recovery record and encouraging client testimonials. I had no idea that this would be the pivotal moment in my fight against cryptocurrency theft. Thanks to their expert team, I was able to recover my lost cryptocurrency back. The process was intricate, but Capital Crypto Recovery's commitment to utilizing the latest technology ensured a successful outcome. I highly recommend their services to anyone who has fallen victim to cryptocurrency fraud. For assistance contact [email protected] and on Telegram OR Call Number +1 (336)390-6684 via email: [email protected] you can visit his website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 24.09.25 15:50 michaeldavenport218

    I was recently scammed out of $53,000 by a fraudulent Bitcoin investment scheme, which added significant stress to my already difficult health issues, as I was also facing cancer surgery expenses. Desperate to recover my funds, I spent hours researching and consulting other victims, which led me to discover the excellent reputation of Capital Crypto Recover, I came across a Google post It was only after spending many hours researching and asking other victims for advice that I discovered Capital Crypto Recovery’s stellar reputation. I decided to contact them because of their successful recovery record and encouraging client testimonials. I had no idea that this would be the pivotal moment in my fight against cryptocurrency theft. Thanks to their expert team, I was able to recover my lost cryptocurrency back. The process was intricate, but Capital Crypto Recovery's commitment to utilizing the latest technology ensured a successful outcome. I highly recommend their services to anyone who has fallen victim to cryptocurrency fraud. For assistance contact [email protected] and on Telegram OR Call Number +1 (336)390-6684 via email: [email protected] you can visit his website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 24.09.25 15:50 michaeldavenport218

    I was recently scammed out of $53,000 by a fraudulent Bitcoin investment scheme, which added significant stress to my already difficult health issues, as I was also facing cancer surgery expenses. Desperate to recover my funds, I spent hours researching and consulting other victims, which led me to discover the excellent reputation of Capital Crypto Recover, I came across a Google post It was only after spending many hours researching and asking other victims for advice that I discovered Capital Crypto Recovery’s stellar reputation. I decided to contact them because of their successful recovery record and encouraging client testimonials. I had no idea that this would be the pivotal moment in my fight against cryptocurrency theft. Thanks to their expert team, I was able to recover my lost cryptocurrency back. The process was intricate, but Capital Crypto Recovery's commitment to utilizing the latest technology ensured a successful outcome. I highly recommend their services to anyone who has fallen victim to cryptocurrency fraud. For assistance contact [email protected] and on Telegram OR Call Number +1 (336)390-6684 via email: [email protected] you can visit his website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 24.09.25 19:08 Raymondkrisitina

    Sylvester Bryant gets my full recommendation. He helped me get back €230,000 in Ethereum when I thought it was gone forever. His skills in tracking and retrieving scam-lost funds amaze me. I appreciated his steady, expert manner and strong tech know-how. That built my trust every step of the way. If a scam has taken your money, get in touch with him right away. Email him at yt7cracker@gmail. com or message on WhatsApp at +1 512 577 7957. He truly commits to aiding folks in regaining their assets. His help meant everything to me.

  • 24.09.25 19:50 Raymondkrisitina

    Sylvester Bryant gets my full recommendation. He helped me get back €230,000 in Ethereum when I thought it was gone forever. His skills in tracking and retrieving scam-lost funds amaze me. I appreciated his steady, expert manner and strong tech know-how. That built my trust every step of the way. If a scam has taken your money, get in touch with him right away. Email him at yt7cracker@gmail. com or message on WhatsApp at +1 512 577 7957. He truly commits to aiding folks in regaining their assets. His help meant everything to me.

  • 24.09.25 19:55 Athea5130

    ETHICAL BITCOIN HACKER/ BEST WAY RECOVERY BITCOIN I believed I was completely lost. I was unable to withdraw money from my investment wallet after making a dubious investment, and until I discovered Best Way Hacker Company, no one could assist me in unfreezing it. This group handled my matter with attention and urgency. In a matter of days, they were able to retrieve my frozen investment and transfer it to a new wallet that I owned. They restored my hope in a big way. Best Way Hacker restored my money and my peace of mind, and for that I will always be grateful. Email.. [email protected] WhatsApp..+39-(350)-967-(1925)

  • 24.09.25 19:56 Athea5130

    ETHICAL BITCOIN HACKER/ BEST WAY RECOVERY BITCOIN I believed I was completely lost. I was unable to withdraw money from my investment wallet after making a dubious investment, and until I discovered Best Way Hacker Company, no one could assist me in unfreezing it. This group handled my matter with attention and urgency. In a matter of days, they were able to retrieve my frozen investment and transfer it to a new wallet that I owned. They restored my hope in a big way. Best Way Hacker restored my money and my peace of mind, and for that I will always be grateful. Email.. [email protected] WhatsApp..+39-(350)-967-(1925)

  • 24.09.25 19:56 Athea5130

    ETHICAL BITCOIN HACKER/ BEST WAY RECOVERY BITCOIN I believed I was completely lost. I was unable to withdraw money from my investment wallet after making a dubious investment, and until I discovered Best Way Hacker Company, no one could assist me in unfreezing it. This group handled my matter with attention and urgency. In a matter of days, they were able to retrieve my frozen investment and transfer it to a new wallet that I owned. They restored my hope in a big way. Best Way Hacker restored my money and my peace of mind, and for that I will always be grateful. Email.. [email protected] WhatsApp..+39-(350)-967-(1925)

  • 24.09.25 19:56 Athea5130

    ETHICAL BITCOIN HACKER/ BEST WAY RECOVERY BITCOIN I believed I was completely lost. I was unable to withdraw money from my investment wallet after making a dubious investment, and until I discovered Best Way Hacker Company, no one could assist me in unfreezing it. This group handled my matter with attention and urgency. In a matter of days, they were able to retrieve my frozen investment and transfer it to a new wallet that I owned. They restored my hope in a big way. Best Way Hacker restored my money and my peace of mind, and for that I will always be grateful. Email.. [email protected] WhatsApp..+39-(350)-967-(1925)

  • 24.09.25 20:52 elizabethrush89

    God bless Capital Crypto Recover Services for the marvelous work you did in my life, I have learned the hard way that even the most sensible investors can fall victim to scams. When my USD was stolen, for anyone who has fallen victim to one of the bitcoin binary investment scams that are currently ongoing, I felt betrayal and upset. But then I was reading a post on site when I saw a testimony of Wendy Taylor online who recommended that Capital Crypto Recovery has helped her recover scammed funds within 24 hours. after reaching out to this cyber security firm that was able to help me recover my stolen digital assets and bitcoin. I’m genuinely blown away by their amazing service and professionalism. I never imagined I’d be able to get my money back until I complained to Capital Crypto Recovery Services about my difficulties and gave all of the necessary paperwork. I was astounded that it took them 12 hours to reclaim my stolen money back. Without a doubt, my USDT assets were successfully recovered from the scam platform, Thank you so much Sir, I strongly recommend Capital Crypto Recover for any of your bitcoin recovery, digital funds recovery, hacking, and cybersecurity concerns. You reach them Call/Text Number +1 (336)390-6684 His Email: [email protected] Contact Telegram: @Capitalcryptorecover Via Contact: [email protected] His website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 24.09.25 20:52 elizabethrush89

    God bless Capital Crypto Recover Services for the marvelous work you did in my life, I have learned the hard way that even the most sensible investors can fall victim to scams. When my USD was stolen, for anyone who has fallen victim to one of the bitcoin binary investment scams that are currently ongoing, I felt betrayal and upset. But then I was reading a post on site when I saw a testimony of Wendy Taylor online who recommended that Capital Crypto Recovery has helped her recover scammed funds within 24 hours. after reaching out to this cyber security firm that was able to help me recover my stolen digital assets and bitcoin. I’m genuinely blown away by their amazing service and professionalism. I never imagined I’d be able to get my money back until I complained to Capital Crypto Recovery Services about my difficulties and gave all of the necessary paperwork. I was astounded that it took them 12 hours to reclaim my stolen money back. Without a doubt, my USDT assets were successfully recovered from the scam platform, Thank you so much Sir, I strongly recommend Capital Crypto Recover for any of your bitcoin recovery, digital funds recovery, hacking, and cybersecurity concerns. You reach them Call/Text Number +1 (336)390-6684 His Email: [email protected] Contact Telegram: @Capitalcryptorecover Via Contact: [email protected] His website: https://recovercapital.wixsite.com/capital-crypto-rec-1

  • 12:29 beverlyalex010

    In 2022, I lost 1.5 ETH in what I thought was a promising Twitter investment. I reached out to the so-called brokers behind it, but they vanished and stopped replying. For years, I carried the heavy belief that my crypto was gone forever. Then, by chance, I discovered RecoveryFundProvider (recoveryfundprovider @ gmail. com WhatsApp +44 736-644-5035) through a finance podcast. That moment changed everything. Their team didn’t lure me in with false promises or flashy guarantees. Instead, they explained their process clearly specializing in advanced blockchain tracing, smart contract forensics, and even transaction reversal in certain cases. Skeptical but hopeful, I allowed them to review my case. What followed was beyond my expectations. They dug deep into the blockchain ledger, followed the coin’s complex movements across multiple addresses, and even collaborated with global nodes to trace every trail. Two weeks later, I received an alert I never thought I’d see again: my crypto had been fully recovered and safely transferred back to me. I cried tears of relief. What sets RecoveryFundProvider apart isn’t just their cutting-edge expertise, but their unwavering ethics and dedication. They treat every client like more than just another case number even years after a loss. Today, I can confidently say they live up to their name, and i am endlessly grateful.

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