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Н Новости

ИИ и прогнозирование микроэлементов для борьбы с загрязнением воды

В последние десятилетия угроза загрязнения водных ресурсов стала одной из наиболее беспокоящих экологических проблем. С ростом мирового потребления фармацевтических препаратов в 2020 году оно достигло 4 миллиардов доз, и как следствие, водные системы сталкиваются с увеличением количества и разнообразия микроэлементов, попадающих в очистные сооружения. Эти вещества, часто неизвестные и трудно поддающиеся анализу, могут оказать вредное воздействие на окружающую среду и здоровье человека, включая канцерогенез и эндокринные нарушения.

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В условиях, где традиционные методы анализа требуют дорогостоящего оборудования, опытных специалистов и затрат времени, наука стремится к эффективным и инновационным подходам. В этом контексте исследовательская команда Корейского Института Науки и Технологии (KIST), под руководством Хон Сок-вона, главы Центра исследования водных ресурсов и цикла, и старшего исследователя Сон Муна, представляет новаторскую методологию, основанную на искусственном интеллекте (AI), для борьбы с вызовами загрязнения воды.

В данной статье мы рассмотрим уникальный подход команды KIST, объединяющий в себе методы самоорганизующихся карт (SOM) для кластеризации и случайных лесов (RFC) в машинном обучении для прогнозирования свойств и поведения микроэлементов. Результаты этого исследования проливают свет на возможности применения искусственного интеллекта в экологии, предоставляя быстрый и точный инструмент для анализа и прогнозирования воздействия микроэлементов в водных системах.

Приятного прочтения(:

Кластеризация с самоорганизующимися картами (SOM)

Обучение самоорганизующихся карт (SOM)

С использованием физико-химических свойств, функциональных групп и биотрансформационных правил в качестве входных параметров, SOM были обучены выделять схожие особенности между трассовыми веществами. Процесс обучения позволил картам выстраивать кластеры, где элементы внутри кластера демонстрировали схожие характеристики.

Развитие SOM: credit
Развитие SOM: credit

Выделим ключевые шаги:

  • Инициализация: Процесс начинается с инициализации карты, представляющей изначальный вес каждого нейрона. Эти веса обычно устанавливаются случайным образом или используются некоторые методы, например, PCA (Principal Component Analysis).

  • Выборка данных: Затем из обучающего набора данных случайным образом выбирается вектор. Этот вектор является примером из пространства входных данных, который будет использоваться для коррекции весов нейронов.

  • Конкуренция: Выбранный вектор сравнивается с весами всех нейронов, и нейрон с наилучшим соответствием (наименьшим расстоянием) считается победителем. Этот нейрон называется "победителем сети".

  • Адаптация весов: Веса всех нейронов адаптируются в направлении вектора обучения, сосредотачиваясь вокруг победителя. Этот процесс приводит к тому, что соседние нейроны в пространстве карты начинают представлять схожие характеристики.

  • Итерации: Эти шаги повторяются множество раз для различных векторов из обучающего набора данных. Каждая итерация приближает SOM к описанию структуры входных данных.

  • Снижение размерности: В процессе обучения размерность карты обычно уменьшается, что позволяет сжать информацию о данных и выделить главные черты их структуры.

Кластеризация и группировка микроэлементов

Получившиеся SOM-карты обеспечили интуитивно понятное представление о структуре данных, где цветовое кодирование и близость элементов на карте отражали степень их схожести. Это сделало процесс группировки микроэлементов более понятным и визуально доступным для дальнейшего анализа.

Определение маркерных компонентов

Ward’s метод использовался для определения границ между кластерами на SOM-картах. Этот подход выявил маркерные компоненты в каждом кластере, обеспечивая представление о типичных представителях данной группы. Маркерные компоненты стали ключевыми элементами в дальнейшем прогнозировании свойств и концентраций микроэлементов.

Процесс Варда в кластеризации SOM:

  • Обучение SOM: Сначала проводится обучение Самоорганизующихся Карт на входных данных, включающих физико-химические свойства, функциональные группы и биотрансформационные правила микроэлементов.

  • Вычисление расстояний: После обучения проводится расчет расстояний между узлами карты. Эти расстояния используются для определения степени схожести между кластерами.

  • Формирование кластеров: Метод Варда выстраивает кластеры, объединяя узлы карты с наименьшими значениями расстояний между ними. Это происходит с учетом минимизации общего расстояния между всеми узлами.

  • Оптимизация кластеров: Процесс объединения кластеров продолжается до тех пор, пока не достигнута оптимальная структура, при которой внутрикластерное расстояние минимально, а межкластерное максимально.

Математическая формула метода Варда:

Для каждого шага метода Варда используется следующая формула для вычисления расстояния между кластерами:

Dij=ninjni+njdij2

где:

Dij – расстояние между кластерами i и j,
ni, nj – количество узлов в соответствующих кластерах,

dij– евклидово расстояние между центроидами кластеров.

Преимущества метода Варда в кластеризации SOM:

  • Четкость границ: Метод Варда способствует формированию компактных и четко выделенных кластеров.

  • Учет расстояний: Значение расстояния учитывает внутрикластерные и межкластерные взаимодействия, обеспечивая баланс между компактностью и различимостью.

Применение SOM для классификации

С учетом обученных SOM и маркерных компонентов, мы можем классифицировать 13 новых трассовых веществ с высокой точностью. Карта SOM служила своеобразной "картой памяти", позволяя предсказывать свойства и концентрации новых микроэлементов, основываясь на их сходстве с ранее исследованными компонентами.

Преимущества кластеризации с SOM

Этот подход к кластеризации с использованием SOM обладает несколькими преимуществами. Во-первых, он позволяет визуализировать и понять структуру данных, что облегчает интерпретацию результатов. Во-вторых, маркерные компоненты предоставляют конкретные представления о характеристиках каждого кластера. Эти преимущества сделали SOM мощным инструментом в первичном этапе анализа трассовых веществ и обеспечили основу для последующего машинного обучения.

Листинг кода для обучения SOM

Представим пример кода с использованием библиотеки MiniSOM, которая предоставляет реализацию самоорганизующихся карт:

from minisom import MiniSom

import numpy as np

# Загрузка данных для обучения SOM (пример)

data = np.random.rand(100, 5) # Здесь необходимо загрузить фактические данные

# Определение размеров карты

map_size = (10, 10)

# Создание и обучение SOM

som = MiniSom(map_size[0], map_size[1], data.shape[1], sigma=0.5, learning_rate=0.5)

som.random_weights_init(data)

som.train_random(data, 1000, verbose=True)

# Получение карты после обучения

trained_map = som.get_weights()

# Дополнительные шаги могут включать визуализацию карты или использование ее для кластеризации данных

В реальном исследовании код, вероятно, был бы более сложным и включал бы дополнительные шаги для предобработки данных, настройки параметров SOM и анализа результатов.

Математическая модель SOM

Математически, Самоорганизующиеся Карты (SOM) представляют собой искусственные нейронные сети, организованные в двумерную сетку, предназначенную для кластеризации данных. Процесс обучения SOM связан с поиском оптимальных весов между нейронами, чтобы точнее представить структуру входных данных. Математическая модель SOM выражается следующей системой уравнений:

  1. Инициализация весов:

Wi,j=Xrand,

где Wi,j – веса нейрона на позиции (i, j), а Xrand – случайно выбранный вектор из входных данных.

  1. Вычисление близости:

di,j=nk=1(Xk-Wi,j,k)2,

где di,j – мера близости между входным вектором Х и весами нейрона Wi,j, n – размерность входных данных.

  1. Определение победителя:

(iwin,jwin) =argi,jmindi,j,

где (iwin,jwin) – координаты нейрона, который обладает наименьшей близостью к входному вектору.

  1. Обновление весов:

Wновi,j=Wстарi,j+hi,j(X-Wстарi,j),

где – коэффициент обучения, hi,j – функция окрестности, определяющая степень влияния на соседние нейроны.

Эти уравнения описывают основной процесс обучения SOM, который нацелен на организацию данных в пространстве таким образом, чтобы схожие элементы оказывались близкими по координатам на SOM-карте.

Химический контекст

В области исследований микроэлементов и микрозагрязнителей, химический контекст выступает важным аспектом, который определяет взаимодействие веществ и их дальнейшие судьбы в природной среде. Самоорганизующиеся карты (SOM) в этом контексте предоставляют мощный инструмент для анализа и классификации химических соединений.

  • Физико-химические свойства: SOM, обученные на физико-химических характеристиках микроэлементов, способны выявлять схожие особенности в их структуре. Например, водорастворимость, коэффициенты распределения в системе октанол-вода (log Kow), и другие параметры могут служить основой для группировки веществ схожей природы.

  • Функциональные группы: SOM прекрасно подходят для выделения взаимосвязи между веществами, содержащими определенные функциональные группы. Например, аминокислоты, содержащие аминогруппу, или эфиры с определенными биотрансформационными маршрутами, могут быть эффективно кластеризованы на SOM-карте.

  • Биотрансформационные правила: Обучение SOM на основе биотрансформационных правил добавляет в модель знание о том, как микроэлементы претерпевают изменения под воздействием биологических процессов. Это включает в себя процессы, такие как гидролиз, окисление, деметилирование и другие, которые могут влиять на устойчивость вещества в окружающей среде.

Прогнозирование со случайными лесами (RFC)

Билдинг и оптимизация RFC

Процесс построения и оптимизации модели с использованием классификации случайного леса (Random Forest Classifier, RFC) представляет собой критический этап в обеспечении точности и эффективности прогнозирования микроэлементов. Давайте рассмотрим этот процесс в деталях.

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Листинг кода

В данном разделе представлен листинг кода, отражающий реализацию алгоритма RFC для классификации микроэлементов. Далее представлены ключевые шаги, выполняемые в ходе построения и оптимизации модели.

from sklearn.ensemble import RandomForestClassifier

from sklearn.model_selection import train_test_split

from sklearn.metrics import accuracy_score

# Разделение данных на тренировочные и тестовые

X_train, X_test, y_train, y_test = train_test_split(features, labels, test_size=0.2, random_state=42)

# Инициализация и настройка RFC модели

rfc_model = RandomForestClassifier(n_estimators=100, random_state=42)

# Обучение модели

rfc_model.fit(X_train, y_train)

# Предсказание на тестовых данных

predictions = rfc_model.predict(X_test)

# Оценка точности модели

accuracy = accuracy_score(y_test, predictions)

Разбор кода и шагов

  • Разделение данных: Первый шаг включает разделение данных на тренировочные (X_train, y_train) и тестовые (X_test, y_test) наборы с использованием train_test_split.

  • Инициализация и настройка модели: Модель RFC инициализируется с использованием RandomForestClassifier с параметром n_estimators=100, указывая количество деревьев в лесу. Этот параметр можно настраивать в зависимости от конкретных требований.

  • Обучение модели: Происходит обучение модели на тренировочных данных с использованием fit.

  • Предсказание на тестовых данных: Модель предсказывает метки классов для тестовых данных с использованием predict.

  • Оценка точности модели: Точность модели оценивается сравнением предсказанных меток с фактическими метками тестовых данных, используя accuracy_score.

Немного математики

Метод RFC основан на ансамбле решающих деревьев. Вероятность классификации объекта x в RFC определяется как голосование деревьев:

P(y|x)=1NNi=1I(fi(x)=y),

где:

  • P(y|x) – вероятность класса y для объекта x,

  • N – количество деревьев в лесу,

  • fi(x) – прогноз i-го дерева для объекта x,

  • I() – индикаторная функция.

Построение дерева решений

Каждое дерево RFC строится с использованием подвыборки данных и случайного выбора признаков на каждом разделении. Критерий, такой как "индекс Джини" или "энтропия", используется для измерения качества разделения в узлах дерева.

Признаки выбираются случайным образом, что обеспечивает разнообразие между деревьями и уменьшает переобучение.

Больше математических концепций можно найти в библиотеке Scikit-learn.

Преимущества Использования RFC

  • Ансамблевый подход: RFC объединяет прогнозы нескольких деревьев, что улучшает устойчивость модели.

  • Гибкость и универсальность: Модель RFC применима для задач классификации и регрессии.

  • Устойчивость к переобучению: Благодаря случайному выбору признаков на каждом шаге, RFC устойчив к переобучению.

Оптимизация Параметров

Оптимальные параметры RFC, такие как количество деревьев (n_estimators) и глубина деревьев (max_depth), могут быть настроены с использованием кросс-валидации или других методов оптимизации для достижения наилучшей производительности модели.

Кластеризация на основе физико-химических свойств и групп функциональных элементов

Анализ свойств функциональных групп

В контексте исследования, анализ физико-химических свойств и функциональных групп микроэлементов представляет собой важный этап, направленный на выделение схожих характеристик между молекулами. Этот этап играет ключевую роль в создании основы для последующей кластеризации и классификации микроэлементов.

Пример использования

# Пример использования SOM для кластеризации

from minisom import MiniSom

# Инициализация SOM

som = MiniSom(x=5, y=5, input_len=len(features.columns), sigma=1.0, learning_rate=0.5)

# Обучение SOM на физико-химических свойствах и функциональных группах

som.random_weights_init(features.values)

som.train_batch(features.values, num_iteration=1000)

# Получение карты кластеров

cluster_map = som.labels_map(features.values)

В данном листинге кода:

  • MiniSom представляет реализацию мини-самоорганизующейся карты,

  • som.random_weights_init инициализирует веса SOM на основе физико-химических свойств и функциональных групп,

  • som.train_batch обучает SOM на данных.

Группировка по физико-химическим характеристикам

Физико-химические Свойства:

  • Гидрофобность (Octanol-Water Partitioning Coefficient): Одним из важных физико-химических параметров является гидрофобность молекулы, выраженная коэффициентом разделения октанол-вода. Этот параметр определяет способность микроэлемента к разделению между органической и водной фазами.

  • Функциональные группы: Анализ наличия определенных функциональных групп, таких как эфир, эстеры и амины, может дополнительно характеризовать молекулярные свойства. Например, аминогруппы могут указывать на биологическую активность.

Функциональные Группы:

  • Эфиры: Молекулы, содержащие эфирные группы, могут обладать особыми свойствами, такими как устойчивость к биологическому разложению или специфическое взаимодействие с микроорганизмами.

  • Эстеры: Наличие эфирных групп может влиять на реакцию биотрансформации микроэлементов, а также на их токсичность.

  • Амины: Аминогруппы могут быть связаны с биологической активностью и влиять на процессы разложения водных сточных вод.

Анализ этих свойств и функциональных групп позволяет эффективно разделить микроэлементы на группы схожих характеристик. Этот этап подготавливает почву для последующего использования методов машинного обучения, таких как кластеризация с самоорганизующимися картами и прогнозирование с использованием случайных лесов.

Применение методов кластеризации и визуализации

В этом разделе мы рассмотрим процесс применения методов кластеризации, в частности, самоорганизующихся карт (SOM), и визуализации результатов для эффективного анализа микроэлементов.

Кластеризация с Самоорганизующимися Картами (SOM)

  • Выбор входных параметров: Для обучения SOM используются физико-химические свойства, функциональные группы и биотрансформационные правила микроэлементов в качестве входных параметров. Это позволяет учесть разнообразные характеристики молекул и определить схожие черты между ними.

# Пример использования физико-химических свойств, функциональных групп и биотрансформационных правил в качестве входных параметров для SOM

som_input = prepare_som_input(physical_properties, functional_groups, biotransformation_rules)

  • Обучение SOM: Самоорганизующиеся карты обучаются на подготовленных данных, выстраивая кластеры микроэлементов в пространстве с учетом их схожести.

# Инициализация и обучение SOM

som_model = SelfOrganizingMap()

som_model.train(som_input)

  • Выделение кластеров: После обучения SOM каждый кластер представляет собой группу микроэлементов с схожими характеристиками.

# Получение кластеров из обученной SOM

clusters = som_model.get_clusters()

Визуализация Результатов

  • Карта кластеров: Визуализация самоорганизующихся карт позволяет наглядно представить структуру кластеров и взаимное распределение микроэлементов.

# Визуализация карты кластеров

visualize_som_clusters(clusters)

  • Характеристики кластеров: После выделения кластеров важно проанализировать характеристики микроэлементов в каждом кластере.

# Анализ характеристик микроэлементов в каждом кластере

analyze_cluster_characteristics(clusters)

Визуализация этих результатов существенна для понимания внутренней структуры данных, выявления закономерностей и последующего использования этой информации в моделях машинного обучения для прогнозирования поведения микроэлементов и классификации новых данных.

Кластеризация на основе биотрансформационных правил

В этом разделе мы более детально рассмотрим процесс кластеризации микроэлементов на основе биотрансформационных правил и проведем сравнение с физико-химическим подходом.

Анализ и группировка на основе биотрансформации

Этап анализа и группировки микроэлементов на основе биотрансформации является ключевым в контексте прогнозирования их поведения в водных системах. Здесь рассматриваются биологические механизмы взаимодействия среды с микроэлементами, что позволяет учесть их динамические изменения под воздействием организмов. Такой подход отражает реальные процессы, происходящие в природных условиях.

Метод биотрансформации позволяет выявить, какие элементы подвергаются изменениям в результате метаболизма биологических систем. Это важно для понимания, каким образом микроэлементы взаимодействуют с организмами и какие метаболиты образуются в процессе их обработки. Группировка на основе биотрансформации обеспечивает более точное представление о долгосрочных эффектах микроэлементов на экосистемы водных объектов.

  • Подготовка Данных: Входные данные включают биотрансформационные правила для каждого микроэлемента. Эти правила предоставляют информацию о возможных биологических превращениях.

# Подготовка данных для кластеризации на основе биотрансформационных правил

biotransformation_data = prepare_biotransformation_data(microelements, biotransformation_rules)

  • Кластеризация с использованием RFC: Random Forest Classifier (RFC) применяется для классификации микроэлементов на основе их биотрансформационных правил.

# Инициализация и обучение RFC модели на биотрансформационных данных

rfc_model_biotransformation = RandomForestClassifier(n_estimators=100, random_state=42)

rfc_model_biotransformation.fit(X_biotransformation_train, y_biotransformation_train)

Сравнение с физико-химическим подходом

В сравнении с традиционными физико-химическими методами, фокусирующимися на свойствах вещества и его взаимодействии с окружающей средой, биотрансформационный подход призван улучшить прогнозы в условиях, приближенных к реальным. Физико-химические методы не всегда учитывают сложные биологические механизмы, происходящие в системе, и могут оставаться ограниченными в способности предсказать последствия воздействия микроэлементов на живые организмы.

Важно отметить, что совмещение биотрансформационных и физико-химических подходов может предложить комплексный взгляд на поведение микроэлементов. Такие интегрированные методы обеспечивают более глубокое понимание процессов и позволяют уточнить прогнозы, что в конечном итоге важно для разработки эффективных стратегий управления качеством воды и снижения негативного воздействия на окружающую среду.

Представим два рисунка, демонстрирующие результаты кластеризации на основе:

  1. физико-химического подхода и функциональных групп микрозагрязнителей,

  2. биотрансформационных правил.

Карты расстояний, полученные из SOM, иллюстрированы разными цветами в зависимости от относительного расстояния между каждым нейроном карты(а). Микрозагрязнители (MPs) интерпретировались как “с аналогичными характеристиками”, если размещались близко на карте расстояний.

8f2f9a2e2c896b267f326ba6234254aa.png0f2ededd58b776cfb8ebae95d5270aa9.png

*Палитра показывает расстояние между соседними единицами карты

Машинное обучение в прогнозировании новых микроэлементов

6cc257996bf54fd748d03784ce42e5bd.png

В условиях постоянного увеличения потребления фармацевтических препаратов, важно иметь инструменты, способные предсказывать поведение новых микроэлементов в водных системах. Исследование сосредотачивается на применении методов машинного обучения для решения этой проблемы. Ключевым этапом является оценка точности и эффективности предложенной модели.

Оценка точности и эффективности

Модель была подвергнута тщательной оценке с целью определения ее точности в прогнозировании и общей эффективности.

1. Оценка Точности:

  • Производится анализ точности модели, измеренной через метрики, такие как accuracy score.

from sklearn.metrics import accuracy_score

# Оценка точности модели на тестовых данных

accuracy_model = accuracy_score(y_test, predictions_model)

print(f"Accuracy: {accuracy_model}")

2. Эффективность Модели:

  • Изучение эффективности модели в прогнозировании характеристик микроэлементов.

# Предсказание на тестовых данных

predictions_model = model.predict(X_test)

# Анализ эффективности модели

efficiency_analysis = analyze_model_efficiency(y_test, predictions_model)

print(f"Model Efficiency: {efficiency_analysis}")

Применение RFC к 13 новым микроэлементам

Для проверки эффективности модели, RFC был успешно применен к 13 новым микроэлементам. Этот этап исследования был важен для проверки способности модели предсказывать поведение и свойства ранее неизвестных микроэлементов. Результаты показали, что модель смогла достичь высокой точности прогнозирования, приблизительно 75%. Это свидетельствует о том, что модель успешно обобщает свои знания на новые микроэлементы, обеспечивая точные предсказания, даже в условиях ограниченной информации.

Сравнение с традиционными методами

Для оценки превосходства предложенной модели проведено сравнение с традиционными методами. Подход, использующий машинное обучение с использованием RFC, был сравнен с традиционными методами, основанными на биологической информации. Результаты указывают на значительное преимущество модели машинного обучения: точность ее прогнозов составила около 75%, в то время как традиционные методы обеспечивали точность порядка 40%. Таким образом, предложенная модель не только превосходит традиционные методы, но и значительно повышает точность прогнозов, что делает ее важным инструментом для предсказания свойств и поведения микроэлементов в водных системах.

Преимущества ИИ в точности прогнозирования

Искусственный интеллект в данной модели демонстрирует высокую точность в прогнозировании свойств микроэлементов. Это достигается благодаря использованию сложных алгоритмов машинного обучения, таких как случайные леса (RFC), способных выявлять нелинейные зависимости в данных. Точность в предсказаниях оказывает влияние на эффективность принимаемых решений в области водных технологий и экологической безопасности.

Анализ применения к реальным ситуациям

Применение разработанной модели к реальным ситуациям подтвердило ее практическую применимость. Модель эффективно предсказывала поведение микроэлементов, а результаты соответствовали данным, полученным в реальных условиях сточных вод и водных систем. Например, при анализе влияния фармацевтических микроэлементов, точность прогнозирования в аналогичных условиях сравнительно высока, что подтверждает преимущества предложенной модели.

Однако использование модели не ограничивается только научными исследованиями. Ее прикладное использование может охватывать области водных и сточных систем, предоставляя быструю и точную информацию о микроэлементах. Перспективы включают в себя расширение области применения модели на различные типы веществ и условия окружающей среды, а также дальнейшее совершенствование методов обучения.

В итоге, использование ИИ, такого как разработанная модель, в прогнозировании микроэлементов предоставляет современный и эффективный инструмент для экологических и инженерных приложений в области водных ресурсов.

Заключение

Результаты проведенного исследования подчеркивают важность применения искусственного интеллекта (ИИ), особенно в виде разработанной модели AI KIST, для прогнозирования поведения микроэлементов в водных системах. Сочетание методов кластеризации и машинного обучения позволяет не только эффективно классифицировать известные микроэлементы, но также успешно прогнозировать свойства новых веществ.

Влияние и инновации AI KIST

AI KIST представляет собой инновационный подход к решению проблем загрязнения воды. Модель, основанная на таких алгоритмах как, самоорганизующиеся карты Кохонена и случайных лесах, демонстрирует превосходство в прогнозировании, что имеет важное значение для управления качеством воды и охраны окружающей среды. Разработанная методология становится инструментом для эффективного контроля за воздействием микроэлементов.

Дальнейшие шаги и возможное развитие

Несмотря на успешные результаты, исследование оставляет множество возможностей для дальнейшего развития. В следующих этапах исследования можно углубить анализ воздействия микроэлементов на биологические системы и продолжить расширение базы данных для повышения точности прогнозирования. Важно также обратить внимание на адаптацию модели к различным географическим и климатическим условиям.

Как мне кажется, с постоянным увеличением глобального потребления фармацевтических препаратов неотложной становится задача эффективного контроля и обработки сточных вод, содержащих следы этих веществ. Модель, представленная в данном исследовании, предоставляет перспективу для широкого внедрения в системы водоочистки и мониторинга, способствуя оптимизации процессов и минимизации экологических рисков.

Таким образом, с учетом динамики роста фармацевтической индустрии и повышенного внимания к экологическим проблемам, прогностический потенциал модели AI KIST становится ключевым фактором в обеспечении устойчивого управления водными ресурсами в будущем.

Резюмируем: использование AI KIST не только улучшает наше понимание водных систем, но и предоставляет эффективные инструменты для борьбы с вызовами, связанными с загрязнением воды. Это внедрение инновационных методов в практику, направленных на устойчивое управление водными ресурсами и снижение негативного воздействия на окружающую среду.

Я была очень рада подготовить этот материал, поскольку тема действительно крайне важна в наше время, поэтому с удовольствием почитаем фидбэк в комментариях, спасибо(:

Автор: Вероника Веселова

Источник

  • 07.09.23 16:24 CherryTeam

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  • 17.07.24 02:26 thompsonrickey

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  • 06.09.24 01:35 Celinagarcia

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  • 06.09.24 01:44 Celinagarcia

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  • 23.09.24 18:56 matthewshimself

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  • 02.10.24 22:27 Emily Hunter

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  • 31.10.24 00:13 ytre89

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  • 12.11.24 00:50 TERESA

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  • 21.11.24 08:02 Emily Hunter

    If I hadn't found a review online and filed a complaint via email to support@deftrecoup. com , the people behind this unregulated scheme would have gotten away with leaving me in financial ruins. It was truly the most difficult period of my life.

  • 22.11.24 04:41 [email protected]

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